Immunohistochemical identification of the beta(3)-adrenoceptor in intact human adipocytes and ventricular myocardium: effect of obesity and treatment with ephedrine and caffeine
De Matteis R, Arch JR, Petroni ML, Ferrari D, Cinti S, Stock MJ
International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity · 61 citas
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Cómo se estudió
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- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2002-11-01 · Int J Obes Relat Metab Disord · vol. 26 · número 11 · págs. 1442–1450
- Editorial
- Springer Nature
- Citado
- 85 citas · más que el 81% de estudios similares · 1.5× el promedio de su campo
- Referencias
- 36 trabajos
- Acceso
- De pago
- Áreas de investigación
- Adipose Tissue and Metabolism · Pharmacology and Obesity Treatment · Adipokines, Inflammation, and Metabolic Diseases
- Palabras clave
- beta3-adrenergic receptor, white adipocytes, brown adipocytes, obesity, ephedrine, caffeine, lipolysis, ventricular myocardium, receptor subcellular distribution, pheochromocytoma, type 2 diabetes, negative inotropic effect, hypocaloric diet
- MeSH
- myocardium, heart ventricles, adipose tissue, adipocytes, humans, obesity, morbid, ephedrine, caffeine, receptors, adrenergic, beta-3, adrenergic beta-agonists, immunohistochemistry, adolescent, adult, female, male
6 autores
- Rita De Matteis
- Jonathan R.S. Arch🇬🇧GlaxoSmithKline (United Kingdom)
- Maria Letizia Petroni🇮🇹IRCCS Istituto Auxologico Italiano
- D Ferrari🇮🇹IRCCS Istituto Auxologico Italiano
- Saverio Cinti🇬🇧 · autor de correspondenciaUniversity of Buckingham
- MJ Stock🇬🇧St George's, University of London
Resumen
El texto original del estudio, en el idioma en que se publicó.
Objectives
To investigate whether the beta(3)-adrenoceptor could be identified by immunohistochemistry in intact human white and brown adipocytes and other human tissues, and to investigate the influence of obesity and its treatment with ephedrine and caffeine on the expression of the beta(3)-adrenoceptor in adipocytes.
Methods
Morbidly obese patients were given a hypoenergetic diet (70% of energy expenditure) and some were also treated with ephedrine and caffeine (20/200 mg, three times daily) for 4 weeks. Adipose tissue and other tissues were taken during surgery. Immunohistochemistry was carried out using a monoclonal antibody raised against the human beta(3)-adrenoceptor.
Results
Staining was localized to the periphery of cells. All white adipocytes were stained. Those from lean subjects and obese subjects treated with ephedrine and caffeine showed more intense staining than those from untreated obese subjects. Staining was more intense in brown than in white adipocytes in perirenal adipose tissue from phaeochromocytoma patients. Staining was also seen in ventricular myocardium, and in smooth muscle of the prostate, ileum, colon and gall bladder.
Discussion
The tissue and subcellular distribution of staining was consistent with it being due to binding of the antibody to the human beta(3)-adrenoceptor. The presence of the beta(3)-adrenoceptor in human white adipocytes is consistent with evidence that it can mediate lipolysis in human white adipocytes. The increased expression of the beta(3)-adrenoceptor in obese subjects treated with caffeine and ephedrine supports the potential of beta(3)-adrenoceptor agonists in the treatment of obesity and type 2 diabetes. Its expression in ventricular myocardium is consistent with evidence that the beta(3)-adrenoceptor mediates a negative inotropic effect in this tissue.
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