AMP kinase expression and activity in human skeletal muscle: effects of immobilization, retraining, and creatine supplementation
Eijnde BO, Derave W, Wojtaszewski JF, Richter EA, Hespel P
Journal of applied physiology (Bethesda, Md. : 1985) · 12 citas
Etiquetas de revisión
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2004-11-01 · J Appl Physiol (1985) · vol. 98 · número 4 · págs. 1228–1233
- Editorial
- American Physiological Society
- Citado
- 23 citas · más que el 61% de estudios similares · 0.5× el promedio de su campo
- Referencias
- 36 trabajos
- Acceso
- De pago
- Áreas de investigación
- Metabolism, Diabetes, and Cancer · Muscle metabolism and nutrition · Muscle Physiology and Disorders
- Palabras clave
- AMPK, skeletal muscle, muscle immobilization, creatine supplementation, muscle retraining, weight training, vastus lateralis, muscle inactivity, rehabilitation
- MeSH
- muscle, skeletal, humans, creatine, adenylate kinase, exercise, administration, oral, double-blind method, immobilization, gene expression regulation, enzyme activation, weight-bearing, dietary supplements, adolescent, adult, female, male, physical exertion
5 autores
- Bert O. Eijnde🇧🇪KU Leuven
- Wim Derave
- Jørgen F. P. Wojtaszewski
- Erik Arne Richter
- Peter Hespel
Resumen
El texto original del estudio, en el idioma en que se publicó.
The effects of leg immobilization and retraining in combination with oral creatine intake on muscle AMP-activated protein kinase (AMPK) protein expression and phosphorylation status were investigated. A double-blind trial was performed in young healthy volunteers (n = 22). A cast immobilized the right leg for 2 wk, whereafter the knee-extensor muscles of that leg were retrained for 6 wk. Half of the subjects received creatine monohydrate throughout the study (Cr; from 15 g down to 2.5 g daily), and the others ingested placebo (P; maltodextrin). Before and after immobilization and retraining, needle biopsies were taken from the right and left vastus lateralis muscles. In the right leg of P and Cr, immobilization did not affect AMPK alpha1-, alpha2-, and beta2-subunit expression or AMPK alpha-subunit phosphorylation status. However, irrespective of the treatment received, retraining increased the degree of alpha-subunit phosphorylation by approximately 25% (P <0.05) and increased AMPK alpha1-subunit expression (P <0.05) in both groups. From the start to the end of the study, AMPK subunit protein expression and alpha-subunit phosphorylation status were unchanged in the contralateral control leg. It is concluded that immobilization-induced muscle inactivity for 2 wk does not alter AMPK alpha1-, alpha2-, and beta2-subunit expression or alpha-AMPK phosphorylation status. Furthermore, the present observations indicate that AMPK probably is not implicated in the previously reported beneficial effects of oral creatine supplementation on muscle during immobilization and rehabilitative weight training.
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