Docosahexaenoic acid restores endothelial function in children with hyperlipidemia: results from the EARLY study
Engler MM, Engler MB, Malloy M, Chiu E, Besio D, Paul S, Stuehlinger M, Morrow J, Ridker P, Rifai N, Mietus-Snyder M
International journal of clinical pharmacology and therapeutics · 103 citas
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institute of Nursing Research
- Gobierno
- National Institute of General Medical Sciences
- Gobierno
- National Institute of Diabetes and Digestive and Kidney Diseases
- Gobierno
- National Center for Research Resources
- Gobierno
- NCRR NIH HHS
- Gobierno
- NINR NIH HHS
- Gobierno
- NIGMS NIH HHS
- Gobierno
- NIDDK NIH HHS
- Subvenciones
- National Center for Research Resources (M01 RR 01271); National Institute of General Medical Sciences (GM 15431); National Institute of Nursing Research (NR 04902); National Institute of Diabetes and Digestive and Kidney Diseases (DK 48831)
Según 8 financiadores declarados.
Publicación
- Publicado
- 2004-12-01 · Int J Clin Pharmacol Ther · vol. 42 · número 12 · págs. 672–679
- Editorial
- Dustri-Verlag
- Citado
- 151 citas · más que el 98% de estudios similares · 8.9× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 5 trabajos
- Acceso
- De pago
- Áreas de investigación
- Fatty Acid Research and Health · Cardiovascular Health and Disease Prevention · Nitric Oxide and Endothelin Effects
- Palabras clave
- docosahexaenoic acid, omega-3 supplementation, endothelial function, flow-mediated dilation, familial hypercholesterolemia, familial combined hyperlipidemia, asymmetric dimethylarginine, oxidative stress, inflammation, lipid profile, brachial artery
- MeSH
- endothelium, vascular, humans, hyperlipidemia, familial combined, inflammation, docosahexaenoic acids, triglycerides, arginine, cross-over studies, double-blind method, oxidative stress, phenotype, adolescent, adult, child, hyperlipoproteinemia type ii, cholesterol, ldl, cholesterol, hdl, cholesterol, vldl, hypolipidemic agents, biomarkers
11 autores
- Marguerite M. Engler🇺🇸University of California, San Francisco
- Mary B. Engler🇺🇸University of California, San Francisco
- Mary J. Malloy
- E. Chiu
- D. Besio
- S. Paul
- M Stuehlinger
- Jason D. Morrow
- Paul M. Ridker
- Nader Rifai
- Michele Mietus‐Snyder
Resumen
El texto original del estudio, en el idioma en que se publicó.
Objective
The primary objective of this study was to determine whether the National Cholesterol Education Program Step II (NCEP-II) diet or supplementation with docosahexaenoic acid (DHA) with the diet, affects endothelial function in children with familial hypercholesterolemia (FH) or the phenotype of familial combined hyperlipidemia (FCH). As secondary endpoints, the influence of diet and DHA supplementation on lipid profiles as well as biomarkers for oxidative stress and inflammation, and asymmetric dimethylarginine (ADMA), an endogenous inhibitor of nitric oxide synthase, were all evaluated.
Methods
In a double-blind, placebo-controlled, randomized, crossover study design, 20 children (ages 9-19 years) with FH (n = 12) and FCH (n = 8) received nutritional counseling based on the National Cholesterol Education Program Step II (NCEP-II) and food guide pyramid dietary guidelines for 6 weeks. They were then randomly assigned to supplementation with docosahexaenoic acid (DHA 1.2 g/d) or placebo for 6 weeks, followed by a washout phase of 6 weeks and crossover phase of 6 weeks while continuing the NCEP-II diet. Endothelium-dependent flow-mediated dilation (FMD) of the brachial artery was determined by high-resolution ultrasound. Plasma levels of total cholesterol, triglycerides and lipoprotein classes (LDL, HDL, VLDL) were measured by ultracentrifugation and enzymatic methods, plasma F2 isoprostanes by gas chromatography/mass spectrometry, urinary 8-OH-2' deoxyguanosine by liquid chromatography, high sensitivity C-reactive protein by immunonephelometry and ADMA by liquid chromatography.
Results
FMD increased significantly after DHA supplementation compared to baseline (p < 0.001), diet alone (p < 0.002), placebo (p < 0.012) and washout (p < 0.001) phases of the study without affecting biomarkers for oxidative stress, inflammation or ADMA. DHA supplementation was associated with increased levels of total cholesterol (p < 0.01), LDL- and HDL cholesterol concentrations (p < 0.001) compared to the NCEP-II diet.
Conclusion
This study demonstrates that DHA supplementation restores endothelial-dependent FMD in hyperlipidemic children. The endothelium may thus be a therapeutic target for DHA. This is consistent with a hypothesis of increasing NO bioavailability, with the potential for preventing the progression of early coronary heart disease in high-risk children.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
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