Glutathione S-transferase M1 polymorphism and metabolism of sulforaphane from standard and high-glucosinolate broccoli
Gasper AV, Al-Janobi A, Smith JA, Bacon JR, Fortun P, Atherton C, Taylor MA, Hawkey CJ, Barrett DA, Mithen RF
The American journal of clinical nutrition · 218 citas
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Cómo se estudió
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- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
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- Resultado principal
- Solo mecanismos
- Consumo medido con
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Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2005-12-01 · Am J Clin Nutr · vol. 82 · número 6 · págs. 1283–1291
- Editorial
- Elsevier BV
- Citado
- 317 citas · más que el 94% de estudios similares · 3.6× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 42 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Glutathione Transferases and Polymorphisms · Phytochemicals and Antioxidant Activities
- Palabras clave
- glutathione S-transferase M1, GSTM1 polymorphism, broccoli, glucosinolates, isothiocyanates, GSTM1 null genotype, urinary excretion, chemoprevention
- MeSH
- humans, brassica, neoplasms, thiocyanates, isothiocyanates, sulfoxides, glutathione transferase, glucosinolates, area under curve, risk factors, cross-over studies, double-blind method, polymorphism, genetic, alleles, adolescent, adult, middle aged, female, male
10 autores
- Amy V. Gasper🇬🇧Norwich Research Park; Quadram Institute
- Ahmed Al-janobi🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- Julie A. Smith🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- James Richard Bacon🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- Paul Fortun🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- Clare T. Atherton🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- Moira A. Taylor🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- Christopher J. Hawkey🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- David A. Barrett🇬🇧University of Nottingham; Norwich Research Park; Quadram Institute
- Richard F. Mithen🇬🇧 · autor de correspondenciaUniversity of Nottingham; Norwich Research Park; Quadram Institute
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Broccoli consumption is associated with a reduction in the risk of cancer, particularly in persons with a functional glutathione S-transferase M1 allele, as opposed rotrose whose GSTM1 gene has been deleted. Sulforaphane, the major isothiocyanate derived from 4-methylsulfinylbutyl glucosinolate, is thought to be the main agent conferring protection.
Objective
We compared sulforaphane metabolism in GSTM1-null and GSTM1-positive subjects after they consumed standard broccoli and high-glucosinolate broccoli (super broccoli).
Design
Sixteen subjects were recruited into a randomized, 3-phase crossover dietary trial of standard broccoli, super broccoli, and water. Liquid chromatography linked to tandem mass spectrometry was used to quantify sulforaphane and its thiol conjugates in plasma and urine.
Results
GSTM1-null subjects had slightly higher, but statistically significant, areas under the curve for sulforaphane metabolite concentrations in plasma, a greater rate of urinary excretion of sulforaphane metabolites during the first 6 h after broccoli consumption, and a higher percentage of sulforaphane excretion 24 h after ingestion than did GSTM1-positive subjects. Consumption of high-glucosinolate broccoli led to a 3-fold greater increase in the areas under the curve and maximum concentrations of sulforaphane metabolites in plasma, a greater rate of urinary excretion of sulforaphane metabolites during the first 6 h after consumption, and a lower percentage of sulforaphane excretion after its ingestion than did the consumption of standard broccoli.
Conclusions
GSTM1 genotypes have a significant effect on the metabolism of sulforaphane derived from standard or high-glucosinolate broccoli. It is possible that the difference in metabolism may explain the greater protection that GSTM1-positive persons gain from consuming broccoli. The potential consequences of consuming glucosinolate-enriched broccoli for GSTM1-null and -positive persons are discussed.
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