Consuming broccoli does not induce genes associated with xenobiotic metabolism and cell cycle control in human gastric mucosa
Gasper AV, Traka M, Bacon JR, Smith JA, Taylor MA, Hawkey CJ, Barrett DA, Mithen RF
The Journal of nutrition · 43 citas
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Alimentación controladaSolo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
- Consumo medido con
- Comida provista por los investigadores
Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- Nottingham University Hospitals NHS Trust
- Universidad u hospital
- University of East Anglia
Según 2 financiadores declarados.
Publicación
- Publicado
- 2007-07-01 · J Nutr · vol. 137 · número 7 · págs. 1718–1724
- Editorial
- Elsevier BV
- Citado
- 68 citas · más que el 92% de estudios similares · 3.1× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 61 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Phytochemicals and Antioxidant Activities · Nutrition, Genetics, and Disease
- Palabras clave
- broccoli, sulforaphane, glucosinolates, xenobiotic metabolism genes, gastric mucosa, gene expression, thioredoxin reductase, GCLM, cell cycle regulation, anticarcinogenic activity, dietary intervention, chemoprevention
- MeSH
- gastric mucosa, humans, brassica, glucosinolates, xenobiotics, protein array analysis, diet, gene expression profiling, cell cycle, gene expression regulation, up-regulation, female, male
8 autores
- Amy V. Gasper🇬🇧 · autor de correspondenciaUniversity of Nottingham; Quadram Institute
- Μαρία Τράκα🇬🇧Quadram Institute
- James Richard Bacon🇬🇧Quadram Institute
- Julie A. Smith🇬🇧Queen's Medical Centre
- Moira A. Taylor🇬🇧University of Nottingham
- Christopher J. Hawkey🇬🇧Queen's Medical Centre
- David A. Barrett🇬🇧University of Nottingham
- Richard F. Mithen🇬🇧Quadram Institute
Resumen
El texto original del estudio, en el idioma en que se publicó.
Epidemiological studies suggest that a diet rich in broccoli can reduce the risk of cancer at several sites. The anticarcinogenic activity has been largely attributed to the biological activity of sulforaphane (SF), the isothiocyanate derived from 4-methylsulphinylbutyl glucosinolate, which accumulates in broccoli. SF induces xenobiotic metabolizing genes in both cell cultures and animal models and induces genes associated with cell cycle arrest and apoptosis. However, it is not known whether these genes are induced in humans after consumption of broccoli. Sixteen subjects were recruited into a randomized, 3-phase crossover dietary trial of standard broccoli, high glucosinolate broccoli, and water. Global changes in gene expression that occurred 6 h after consuming broccoli soups or water were quantified in gastric mucosal tissue, using Affymetrix whole genome microarrays (n = 4), and in selected genes by real-time RT-PCR in the other individuals. Consumption of high glucosinolate broccoli resulted in up-regulation of several xenobiotic metabolizing genes, including thioredoxin reductase, aldoketoreductases, and glutamate cysteine ligase modifier subunit, which have previously been reported to be induced in cell and animal models after exposure to SF. Only 1 such gene was significantly up-regulated after consumption of standard broccoli. The consequences of these results in relation to the potential anticarcinogenic action of broccoli are discussed.
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