Global and targeted gene expression and protein content in skeletal muscle of young men following short-term creatine monohydrate supplementation
Safdar A, Safdar A, Yardley NJ, Snow R, Melov S, Tarnopolsky MA
Physiological genomics · 92 citas
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- Natural Sciences and Engineering Research Council of Canada
Según 1 financiador declarado.
Publicación
- Publicado
- 2007-10-24 · Physiol Genomics · vol. 32 · número 2 · págs. 219–228
- Editorial
- American Physical Society
- Citado
- 148 citas · más que el 85% de estudios similares · 2.1× el promedio de su campo
- Referencias
- 109 trabajos
- Acceso
- De pago
- Áreas de investigación
- Muscle metabolism and nutrition · Muscle Physiology and Disorders · Biochemical effects in animals
- Palabras clave
- creatine monohydrate supplementation, skeletal muscle, gene expression, protein content, osmolarity, cell swelling, kinases, satellite cell proliferation, protein synthesis, glycogen synthesis, vastus lateralis, fat-free mass, total body water
- MeSH
- muscle, skeletal, humans, creatine, muscle proteins, rna, messenger, oligonucleotide array sequence analysis, cross-over studies, double-blind method, gene expression profiling, reverse transcriptase polymerase chain reaction, gene expression regulation, time factors, dietary supplements, adult, male
5 autores
- Adeel Safdar🇨🇦McMaster University
- Nicholas J. Yardley🇨🇦McMaster University
- Rodney J. Snow🇦🇺Deakin University
- Simon Melov🇺🇸Buck Institute for Research on Aging
- Mark Andrew Tarnopolsky🇨🇦McMaster University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Creatine monohydrate (CrM) supplementation has been shown to increase fat-free mass and muscle power output possibly via cell swelling. Little is known about the cellular response to CrM. We investigated the effect of short-term CrM supplementation on global and targeted mRNA expression and protein content in human skeletal muscle. In a randomized, placebo-controlled, crossover, double-blind design, 12 young, healthy, nonobese men were supplemented with either a placebo (PL) or CrM (loading phase, 20 g/day x 3 days; maintenance phase, 5 g/day x 7 days) for 10 days. Following a 28-day washout period, subjects were put on the alternate supplementation for 10 days. Muscle biopsies of the vastus lateralis were obtained and were assessed for mRNA expression (cDNA microarrays + real-time PCR) and protein content (Kinetworks KPKS 1.0 Protein Kinase screen). CrM supplementation significantly increased fat-free mass, total body water, and body weight of the participants (P < 0.05). Also, CrM supplementation significantly upregulated (1.3- to 5.0-fold) the mRNA content of genes and protein content of kinases involved in osmosensing and signal transduction, cytoskeleton remodeling, protein and glycogen synthesis regulation, satellite cell proliferation and differentiation, DNA replication and repair, RNA transcription control, and cell survival. We are the first to report this large-scale gene expression in the skeletal muscle with short-term CrM supplementation, a response that suggests changes in cellular osmolarity.
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