Effects of resistance exercise with and without creatine supplementation on gene expression and cell signaling in human skeletal muscle
Deldicque L, Atherton P, Patel R, Theisen D, Nielens H, Rennie MJ, Francaux M
Journal of applied physiology (Bethesda, Md. : 1985) · 86 citas
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- Gobierno
- NIAMS NIH HHS
- Subvenciones
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR49869)
Según 2 financiadores declarados.
Publicación
- Publicado
- 2007-11-29 · J Appl Physiol (1985) · vol. 104 · número 2 · págs. 371–378
- Editorial
- American Physiological Society
- Citado
- 141 citas · más que el 90% de estudios similares · 3.0× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 46 trabajos
- Acceso
- Acceso abierto (copia en repositorio) · OTHER-OA
- Áreas de investigación
- Muscle metabolism and nutrition · Muscle Physiology and Disorders · Exercise and Physiological Responses
- Palabras clave
- creatine supplementation, resistance training, skeletal muscle gene expression, Akt phosphorylation, 4E-BP1 phosphorylation, mTOR signaling, muscle atrophy, atrogin-1, myostatin, calpain-1, p38 MAPK, GLUT4, myosin heavy chain, muscle protein synthesis, muscle protein degradation, interleukin-6
- MeSH
- muscle, skeletal, humans, creatine, mitogen-activated protein kinases, polysaccharides, muscle proteins, rna, messenger, exercise, administration, oral, cross-over studies, double-blind method, signal transduction, gene expression, transcription, genetic, enzyme activation, phosphorylation, muscle contraction, time factors, dietary supplements, adult, male, proto-oncogene proteins c-akt
7 autores
- Louise Deldicque
- Philip James Atherton🇬🇧University of Nottingham
- Rekha S Patel🇬🇧University of Nottingham
- Daniel Theisen🇧🇪UCLouvain
- Henri Nielens🇧🇪UCLouvain
- Michael J. Rennie🇬🇧University of Nottingham
- Marc Francaux🇧🇪UCLouvain
Resumen
El texto original del estudio, en el idioma en que se publicó.
To test the hypothesis that creatine supplementation would enhance the anabolic responses of muscle cell signaling and gene expression to exercise, we studied nine subjects who received either creatine or a placebo (maltodextrin) for 5 days in a double-blind fashion before undergoing muscle biopsies: at rest, immediately after exercise (10 x 10 repetitions of one-leg extension at 80% 1 repetition maximum), and 24 and 72 h later (all in the morning after fasting overnight). Creatine supplementation decreased the phosphorylation state of protein kinase B (PKB) on Thr308 at rest by 60% (P < 0.05) and that of eukaryotic initiation factor 4E-binding protein on Thr37/46 (4E-BP1) by 30% 24 h postexercise (P < 0.05). Creatine increased mRNA for collagen 1 (alpha(1)), glucose transporter-4 (GLUT-4), and myosin heavy chain I at rest by 250%, 45%, and 80%, respectively, and myosin heavy chain IIA (MHCIIA) mRNA immediately after exercise by 70% (all P < 0.05). Immediately after exercise, and independent of creatine, mRNA for muscle atrophy F-box (MAFbx), MHCIIA, peroxisome proliferator-activated receptor gamma coactivator-1alpha, and interleukin-6 were upregulated (60-350%; P < 0.05); the phosphorylation state of p38 both in the sarcoplasm and nucleus were increased (12- and 25-fold, respectively; both P < 0.05). Concurrently, the phosphorylation states of PKB (Thr308) and 4E-BP1 (Thr37/46) were decreased by 50% and 75%, respectively (P < 0.05). Twenty-four hours postexercise, MAFbx, myostatin, and GLUT-4 mRNA expression decreased below preexercise values (-35 to -50%; P < 0.05); calpain 1 mRNA increased 70% 72 h postexercise (P < 0.05) and at no other time. In conclusion, 5 days of creatine supplementation do not enhance anabolic signaling but increase the expression of certain targeted genes.
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