Broccoli consumption interacts with GSTM1 to perturb oncogenic signalling pathways in the prostate
Traka M, Gasper AV, Melchini A, Bacon JR, Needs PW, Frost V, Chantry A, Jones AM, Ortori CA, Barrett DA, Ball RY, Mills RD, Mithen RF
PloS one · 103 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
- Consumo medido con
- No se indica
Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- University of Nottingham
- Gobierno
- Directorate for Biological Sciences
- Gobierno
- Biotechnology and Biological Sciences Research Council
Según 3 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2008-07-01 · PLoS One · vol. 3 · número 7 · pág. e2568
- Editorial
- Public Library of Science
- Citado
- 154 citas · más que el 98% de estudios similares · 6.3× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 51 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Glutathione Transferases and Polymorphisms · Nutrition, Genetics, and Disease
- Palabras clave
- broccoli, cruciferous vegetables, GSTM1 genotype, sulforaphane, isothiocyanates, prostate cancer, gene expression, EGF signaling, insulin signaling, androgen signaling, thiourea, dietary intervention, inflammation, carcinogenesis
- MeSH
- prostate, humans, brassica, prostatic neoplasms, glutathione transferase, diet, risk factors, signal transduction, models, biological, aged, middle aged, male
13 autores
- Μαρία Τράκα🇬🇧Norwich Research Park; Quadram Institute
- Amy V. Gasper🇬🇧Norwich Research Park; Quadram Institute
- Antonietta Melchini🇮🇹🇬🇧University of Messina; Norwich Research Park; Quadram Institute
- James Richard Bacon🇬🇧Norwich Research Park; Quadram Institute
- Paul W. Needs🇬🇧Norwich Research Park; Quadram Institute
- Victoria Jane Frost🇬🇧University of East Anglia
- Andrew Chantry🇬🇧University of East Anglia
- Alexandra M. E. Jones🇬🇧Norwich Research Park; Sainsbury Laboratory
- Catharine A. Ortori🇬🇧University of Nottingham
- David A. Barrett🇬🇧University of Nottingham
- Richard Y. Ball🇬🇧Norfolk and Norwich University Hospital
- Robert Mills🇬🇧Norfolk and Norwich University Hospital
- Richard F. Mithen🇬🇧 · autor de correspondenciaNorwich Research Park; Quadram Institute
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Epidemiological studies suggest that people who consume more than one portion of cruciferous vegetables per week are at lower risk of both the incidence of prostate cancer and of developing aggressive prostate cancer but there is little understanding of the underlying mechanisms. In this study, we quantify and interpret changes in global gene expression patterns in the human prostate gland before, during and after a 12 month broccoli-rich diet.
Methods and findings
Volunteers were randomly assigned to either a broccoli-rich or a pea-rich diet. After six months there were no differences in gene expression between glutathione S-transferase mu 1 (GSTM1) positive and null individuals on the pea-rich diet but significant differences between GSTM1 genotypes on the broccoli-rich diet, associated with transforming growth factor beta 1 (TGFbeta1) and epidermal growth factor (EGF) signalling pathways. Comparison of biopsies obtained pre and post intervention revealed more changes in gene expression occurred in individuals on a broccoli-rich diet than in those on a pea-rich diet. While there were changes in androgen signalling, regardless of diet, men on the broccoli diet had additional changes to mRNA processing, and TGFbeta1, EGF and insulin signalling. We also provide evidence that sulforaphane (the isothiocyanate derived from 4-methylsuphinylbutyl glucosinolate that accumulates in broccoli) chemically interacts with TGFbeta1, EGF and insulin peptides to form thioureas, and enhances TGFbeta1/Smad-mediated transcription.
Conclusions
These findings suggest that consuming broccoli interacts with GSTM1 genotype to result in complex changes to signalling pathways associated with inflammation and carcinogenesis in the prostate. We propose that these changes may be mediated through the chemical interaction of isothiocyanates with signalling peptides in the plasma. This study provides, for the first time, experimental evidence obtained in humans to support observational studies that diets rich in cruciferous vegetables may reduce the risk of prostate cancer and other chronic disease.
Trial registration
ClinicalTrials.gov NCT00535977.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.