Fish-oil supplementation induces antiinflammatory gene expression profiles in human blood mononuclear cells
Bouwens M, van de Rest O, Dellschaft N, Bromhaar MG, de Groot LC, Geleijnse JM, Müller M, Afman LA
The American journal of clinical nutrition · 212 citas
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2009-06-11 · Am J Clin Nutr · vol. 90 · número 2 · págs. 415–424
- Editorial
- Elsevier BV
- Citado
- 335 citas · más que el 100% de estudios similares · 20.2× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 40 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Fatty Acid Research and Health · Edible Oils Quality and Analysis · Aquaculture Nutrition and Growth
- Palabras clave
- fish oil supplementation, eicosapentaenoic acid, docosahexaenoic acid, polyunsaturated fatty acids, peripheral blood mononuclear cells, gene expression profiles, anti-inflammatory activity, NF-κB signaling, eicosanoid biosynthesis, adipogenesis, hypoxia signaling, high oleic sunflower oil, older adults
- MeSH
- leukocytes, mononuclear, humans, inflammation, fatty acids, omega-3, docosahexaenoic acids, eicosapentaenoic acid, fatty acids, unsaturated, fish oils, dna, complementary, rna, microarray analysis, double-blind method, gene expression profiling, polymerase chain reaction, dose-response relationship, drug, dietary supplements, aged, aged, 80 and over, female, male
8 autores
- Mark Bouwens🇳🇱Wageningen University & Research
- Ondine van de Rest🇳🇱Wageningen University & Research
- Neele S. Dellschaft🇳🇱Wageningen University & Research
- Mechteld Grootte Bromhaar🇳🇱Wageningen University & Research
- C.P.G.M. de Groot🇳🇱Wageningen University & Research
- Johanna Marianne Geleijnse🇳🇱Wageningen University & Research
- Michael Müller🇳🇱Wageningen University & Research
- Lydia A. Afman🇳🇱 · autor de correspondenciaWageningen University & Research
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Polyunsaturated fatty acids can have beneficial effects on human immune cells, such as peripheral blood mononuclear cells (PBMCs). However, the mechanisms of action of polyunsaturated fatty acids on immune cells are still largely unknown.
Objective
The objective was to examine the effects of supplementation with the polyunsaturated fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on whole-genome PBMC gene expression profiles, in healthy Dutch elderly subjects participating in a double-blind trial, by using whole-genome transcriptomics analysis.
Design
The subjects were randomly allocated to 1 of 3 groups: 1) consumption of 1.8 g EPA+DHA/d (n = 36), 2) consumption of 0.4 g EPA+DHA/d (n = 37), or 3) consumption of 4.0 g high-oleic acid sunflower oil (HOSF)/d (n = 38). All supplements were given in capsules. Before and after 26 wk of intervention, blood samples were collected. Microarray analysis was performed on PBMC RNA from 23 subjects who received 1.8 g EPA+DHA/d and 25 subjects who received HOSF capsules. Quantitative real-time polymerase chain reaction was performed in all 111 subjects.
Results
A high EPA+DHA intake changed the expression of 1040 genes, whereas HOSF intake changed the expression of only 298 genes. EPA+DHA intake resulted in a decreased expression of genes involved in inflammatory- and atherogenic-related pathways, such as nuclear transcription factor kappaB signaling, eicosanoid synthesis, scavenger receptor activity, adipogenesis, and hypoxia signaling.
Conclusion
These results are the first to show that intake of EPA+DHA for 26 wk can alter the gene expression profiles of PBMCs to a more antiinflammatory and antiatherogenic status. This trial was registered at clinicaltrials.gov as NCT00124852.
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