p-HPEA-EDA, a phenolic compound of virgin olive oil, activates AMP-activated protein kinase to inhibit carcinogenesis
Khanal P, Oh WK, Yun HJ, Namgoong GM, Ahn SG, Kwon SM, Choi HK, Choi HS
Carcinogenesis · 71 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Research Foundation
- Gobierno
- National Research Foundation of Korea
- Gobierno
- Ministry of Education, Science and Technology
- Subvenciones
- National Research Foundation of Korea (R13-2003-009); Ministry of Education, Science and Technology (R13-2003-009)
Según 3 financiadores declarados.
Publicación
- Publicado
- 2011-01-07 · Carcinogenesis · vol. 32 · número 4 · págs. 545–553
- Editorial
- Oxford University Press
- Citado
- 98 citas · más que el 97% de estudios similares · 7.2× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 51 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Inflammatory mediators and NSAID effects · Cholesterol and Lipid Metabolism · Cancer, Lipids, and Metabolism
- Palabras clave
- AMPK, Chemistry, Protein kinase A, Kinase, Phosphorylation, Activator (genetics), Biochemistry, Molecular biology, Cell biology, Biology, Receptor
- MeSH
- cell line, tumor, humans, cell transformation, neoplastic, aldehydes, tetradecanoylphorbol acetate, phenols, plant oils, antineoplastic agents, apoptosis, cell survival, enzyme activation, tumor suppressor protein p53, cyclooxygenase 2, amp-activated protein kinases, olive oil, cyclopentane monoterpenes
8 autores
- Pukar Khanal🇰🇷Chosun University
- Won Keun Oh🇰🇷Chosun University
- H. J. Yun🇰🇷Chosun University
- Gwang Mo Namgoong🇰🇷Chosun University
- Sang‐Gun Ahn🇰🇷Chosun University
- Sang‐Mo Kwon🇰🇷Chosun University
- Hee-Young Choi🇰🇷 · autor de correspondenciaChosun University
- Han Seok Choi🇰🇷 · autor de correspondenciaChosun University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Phenolic constituents of virgin olive oil are reported to have antitumor activity. However, the underlying molecular mechanisms and specific target proteins of virgin olive oil remain to be elucidated. Here, we report that dialdehydic form of decarboxymethyl ligstroside aglycone (p-HPEA-EDA), a phenolic compound of virgin olive oil, inhibits tumor promoter-induced cell transformation in JB6 Cl41 cells and suppress cyclooxygenase-2 (COX-2) and tumorigenicity by adenosine monophosphate-activated protein kinase (AMPK) activation in HT-29 cells. p-HPEA-EDA inhibited 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced phosphorylation of extracellular signal-regulated kinases 1/2 and p90RSK in JB6 Cl41 cells, resulting in the inhibition of cell proliferation, activator protein-1 transactivation and cell transformation promoted by TPA. Moreover, p-HPEA-EDA strongly inhibited the cell viability and COX-2 expression by activation of AMPK activity in HT-29 cells, resulted from depletion of intracellular adenosine triphosphate. p-HPEA-EDA-induced activation of caspase-3 and poly-adenosine diphosphate-ribose polymerase, phosphorylation of p53 (Ser15) and DNA fragmentation in HT-29 cells, leading to apoptosis. Importantly, p-HPEA-EDA suppressed the colony formation of HT-29 cells in soft agar. In contrast, Compound C, an AMPK inhibitor, and Z-DEVD-FMK, a caspase-3 inhibitor, blocked the p-HPEA-EDA-inhibited colony formation in HT-29 cells. In vivo chorioallantoic membrane assay also showed that p-HPEA-EDA-inhibited tumorigenicity of HT-29 cells. These findings revealed that targeted activation of AMPK and inhibition of COX-2 expression by p-HPEA-EDA contribute to the chemopreventive and chemotherapeutic potential of virgin olive oil against colon cancer cells.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.