Cruciferous vegetable supplementation in a controlled diet study alters the serum peptidome in a GSTM1-genotype dependent manner
Brauer HA, Libby TE, Mitchell BL, Li L, Chen C, Randolph TW, Yasui YY, Lampe JW, Lampe PD
Nutrition journal · 25 citas
Etiquetas de revisión
Alimentación controladaSolo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
- Consumo medido con
- Comida provista por los investigadores
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institutes of Health
- Gobierno
- National Cancer Institute
- Gobierno
- NCI NIH HHS
- Subvenciones
- National Cancer Institute (R01 CA 92288); National Cancer Institute (R01CA070913); National Cancer Institute (F31 CA126471); National Cancer Institute (R01CA126205); National Cancer Institute (R56 CA070913)
Según 3 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2011-01-27 · Nutr J · vol. 10 · número 1 · pág. 11
- Editorial
- BioMed Central
- Citado
- 33 citas · más que el 88% de estudios similares · 2.2× el promedio de su campo
- Referencias
- 36 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Glutathione Transferases and Polymorphisms · Genomics, phytochemicals, and oxidative stress · Epigenetics and DNA Methylation
- Palabras clave
- Cruciferous vegetables, Sulforaphane, Clinical nutrition, Genotype, Food science, Medicine, Crossover study, Internal medicine, Biology, Biochemistry, Cancer, Gene
- MeSH
- humans, brassicaceae, vegetables, isothiocyanates, glutathione transferase, peptides, prealbumin, blood proteins, diet, linear models, risk factors, cross-over studies, genotype, adult, female, male, young adult, alpha-2-hs-glycoprotein
9 autores
- Heather Ann Brauer🇺🇸University of Washington
- Tanya Libby🇺🇸Fred Hutch Cancer Center
- Breeana L. Mitchell🇺🇸University of Washington; Fred Hutch Cancer Center
- Lin Li🇺🇸Fred Hutch Cancer Center
- Chu Chen🇺🇸University of Washington; Fred Hutch Cancer Center
- Timothy W. Randolph🇺🇸Fred Hutch Cancer Center
- Yutaka Yasui🇨🇦University of Alberta
- Johanna W. Lampe🇺🇸University of Washington; Fred Hutch Cancer Center
- Paul D. Lampe🇺🇸 · autor de correspondenciaUniversity of Washington; Fred Hutch Cancer Center
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Cruciferous vegetable intake is inversely associated with the risk of several cancers. Isothiocyanates (ITC) are hypothesized to be the major bioactive constituents contributing to these cancer-preventive effects. The polymorphic glutathione-S-transferase (GST) gene family encodes several enzymes which catalyze ITC degradation in vivo.
Methods
We utilized high throughput proteomics methods to examine how human serum peptides (the "peptidome") change in response to cruciferous vegetable feeding in individuals of different GSTM1 genotypes. In two randomized, crossover, controlled feeding studies (EAT and 2EAT) participants consumed a fruit- and vegetable-free basal diet and the basal diet supplemented with cruciferous vegetables. Serum samples collected at the end of the feeding period were fractionated and matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry spectra were obtained. Peak identification/alignment computer algorithms and mixed effects models were used to analyze the data.
Results
After analysis of spectra from EAT participants, 24 distinct peaks showed statistically significant differences associated with cruciferous vegetable intake. Twenty of these peaks were driven by their GSTM1 genotype (i.e., GSTM1+ or GSTM1- null). When data from EAT and 2EAT participants were compared by joint processing of spectra to align a common set, 6 peaks showed consistent changes in both studies in a genotype-dependent manner. The peaks at 6700 m/z and 9565 m/z were identified as an isoform of transthyretin (TTR) and a fragment of zinc α2-glycoprotein (ZAG), respectively.
Conclusions
Cruciferous vegetable intake in GSTM1+ individuals led to changes in circulating levels of several peptides/proteins, including TTR and a fragment of ZAG. TTR is a known marker of nutritional status and ZAG is an adipokine that plays a role in lipid mobilization. The results of this study present evidence that the GSTM1-genotype modulates the physiological response to cruciferous vegetable intake.
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