Absorption and metabolism of olive oil secoiridoids in the small intestine
Pinto J, Paiva-Martins F, Corona G, Debnam ES, Jose Oruna-Concha M, Vauzour D, Gordon MH, Spencer JP
The British journal of nutrition · 74 citas
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- Financiación independiente
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- Fundação para a Ciência e a Tecnologia
Según 1 financiador declarado.
Publicación
- Publicado
- 2011-03-17 · Br J Nutr · vol. 105 · número 11 · págs. 1607–1618
- Editorial
- Cambridge University Press
- Citado
- 93 citas · más que el 92% de estudios similares · 3.3× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 47 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Edible Oils Quality and Analysis · Cholesterol and Lipid Metabolism · Phytochemicals and Antioxidant Activities
- Palabras clave
- Ileum, Chemistry, Jejunum, Homovanillic acid, Glucuronidation, Hydroxytyrosol, Biochemistry, Metabolism, Small intestine, Enzyme
- MeSH
- intestine, small, caco-2 cells, animals, humans, rats, phenols, pyrans, plant oils, molecular structure, biological transport, intestinal absorption, hydrogen-ion concentration, models, biological, olive oil
8 autores
- Joana Pinto🇵🇹🇬🇧Universidade do Porto; University of Reading
- Fátima Paiva‐Martins🇵🇹Universidade do Porto
- Giulia Corona🇬🇧University of Reading
- Edward S. Debnam🇬🇧The Royal Free Hospital; University College London
- Marı́a José Oruña-Concha🇬🇧University of Reading
- David Vauzour🇬🇧University of Reading
- Michael H. Gordon🇬🇧University of Reading
- Jeremy P. E. Spencer🇬🇧 · autor de correspondenciaUniversity of Reading
Resumen
El texto original del estudio, en el idioma en que se publicó.
The secoiridoids 3,4-dihydroxyphenylethanol-elenolic acid (3,4-DHPEA-EA) and 3,4-dihydroxyphenylethanol-elenolic acid dialdehyde (3,4-DHPEA-EDA) account for approximately 55 % of the phenolic content of olive oil and may be partly responsible for its reported human health benefits. We have investigated the absorption and metabolism of these secoiridoids in the upper gastrointestinal tract. Both 3,4-DHPEA-EDA and 3,4-DHPEA-EA were relatively stable under gastric conditions, only undergoing limited hydrolysis. Both secoiridoids were transferred across a human cellular model of the small intestine (Caco-2 cells). However, no glucuronide conjugation was observed for either secoiridoid during transfer, although some hydroxytyrosol and homovanillic alcohol were formed. As Caco-2 cells are known to express only limited metabolic activity, we also investigated the absorption and metabolism of secoiridoids in isolated, perfused segments of the jejunum and ileum. Here, both secoiridoids underwent extensive metabolism, most notably a two-electron reduction and glucuronidation during the transfer across both the ileum and jejunum. Unlike Caco-2 cells, the intact small-intestinal segments contain NADPH-dependent aldo-keto reductases, which reduce the aldehyde carbonyl group of 3,4-DHPEA-EA and one of the two aldeydic carbonyl groups present on 3,4-DHPEA-EDA. These reduced forms are then glucuronidated and represent the major in vivo small-intestinal metabolites of the secoiridoids. In agreement with the cell studies, perfusion of the jejunum and ileum also yielded hydroxytyrosol and homovanillic alcohol and their respective glucuronides. We suggest that the reduced and glucuronidated forms represent novel physiological metabolites of the secoiridoids that should be pursued in vivo and investigated for their biological activity.
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