The effects of low-level laser light exposure on sperm motion characteristics and DNA damage
Firestone RS, Esfandiari N, Moskovtsev SI, Burstein E, Videna GT, Librach C, Bentov Y, Casper RF
Journal of andrology · 38 citas
Etiquetas de revisión
Financiación no declaradaDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2012-05-06 · J Androl · vol. 33 · número 3 · págs. 469–473
- Citado
- 63 citas · más que el 85% de estudios similares · 1.8× el promedio de su campo
- Referencias
- 23 trabajos
- Acceso
- De pago
- Áreas de investigación
- Sperm and Testicular Function · Laser Applications in Dentistry and Medicine · Bee Products Chemical Analysis
- Palabras clave
- Semen, Sperm, DNA fragmentation, Andrology, Sperm motility, Semen analysis, Motility, DNA damage, Chemistry, Biology, Molecular biology, DNA, Apoptosis, Medicine, Biochemistry, Genetics, Programmed cell death
- MeSH
- humans, oligospermia, dna damage, cryopreservation, lasers, infrared rays, dna fragmentation, sperm motility, male, asthenozoospermia
8 autores
- Ross Firestone🇨🇦Mount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute
- Navid Esfandiari🇨🇦Mount Sinai Hospital; University of Toronto; Lunenfeld-Tanenbaum Research Institute
- Sergey I. Moskovtsev🇨🇦University of Toronto; CReATe Fertility Centre
- Eliezer Burstein🇨🇦Mount Sinai Hospital; Lunenfeld-Tanenbaum Research Institute
- German T. Videna🇨🇦CReATe Fertility Centre
- Clifford Lawrence Librach🇨🇦University of Toronto; CReATe Fertility Centre
- Yaakov Bentov🇨🇦Mount Sinai Hospital; University of Toronto; Lunenfeld-Tanenbaum Research Institute
- Robert F. Casper🇨🇦 · autor de correspondenciaMount Sinai Hospital; University of Toronto; Lunenfeld-Tanenbaum Research Institute
Resumen
El texto original del estudio, en el idioma en que se publicó.
The objective of this study was to determine the effects of low-level laser light exposure on the motility of spermatozoa and on DNA damage. Thirty-three semen samples were collected for routine analysis and were classified as normospermic, oligospermic, or asthenospermic. After routine semen analysis was performed, residual semen was divided into treated and control aliquots. Treated samples were exposed to a 30-second infrared laser pulse of 50 mW/cm(2) at 905 nm, a wavelength thought to increase light-sensitive cytochrome c oxidase in the mitochondrial electron transport chain. Samples were then incubated at 37°C, and aliquots were analyzed at 30 minutes and 2 hours using computerassisted semen analysis. After incubation, 250 μL of each sample was frozen at 280°C until DNA fragmentation analysis by flow cytometry. A significant increase in motility, most prominent in oligospermic and asthenospermic samples (85% increase), was observed 30 minutes after the treatment (P < .0001). No significant increase in DNA damage compared with control samples was observed. Significant changes in sperm motion kinetics were observed. Low-level laser light exposure appears to have a positive short-term effect on the motility of treated spermatozoa and did not cause any increase in DNA damage measured at 2 hours. We conclude that some cases of asthenospermia may be related to mitochondrial dysfunction. The implications of this study in terms of future clinical applications needs further investigation.
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