Methylation patterns in sentinel genes in peripheral blood cells of heavy smokers: Influence of cruciferous vegetables in an intervention study
Scoccianti C, Ricceri F, Ferrari P, Cuenin C, Sacerdote C, Polidoro S, Jenab M, Hainaut P, Vineis P, Herceg Z
Epigenetics · 29 citas
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
- Consumo medido con
- No se indica
Quién lo pagó
- Financiación
- Financiación independiente
- Organización sin fines de lucro
- World Cancer Research Fund
- Gobierno
- Medical Research Council
Según 2 financiadores declarados.
Publicación
- Publicado
- 2011-09-01 · Epigenetics · vol. 6 · número 9 · págs. 1114–1119
- Editorial
- Landes Bioscience
- Citado
- 41 citas · más que el 82% de estudios similares · 1.6× el promedio de su campo
- Referencias
- 19 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Epigenetics and DNA Methylation · Family Support in Illness
- Palabras clave
- Biology, DNA methylation, Methylation, Cruciferous vegetables, Epigenetics, Methylenetetrahydrofolate reductase, CDKN2A, Bisulfite sequencing, Carcinogenesis, Physiology, Genetics, Gene, Internal medicine, Cancer, Allele, Gene expression, Medicine
- MeSH
- leukocytes, humans, brassicaceae, isothiocyanates, flavonoids, methylenetetrahydrofolate reductase (nadph2), adaptor proteins, signal transducing, tumor suppressor proteins, nuclear proteins, diet, sequence analysis, dna, smoking, dna methylation, epigenesis, genetic, long interspersed nucleotide elements, genes, p16, middle aged, male, mutl protein homolog 1, glycine max
10 autores
- Chiara Scoccianti🇫🇷Centre international de recherche sur le cancer
- Fulvio Ricceri🇮🇹Italian institute for Genomic Medicine
- Pietro Ferrari🇫🇷Centre international de recherche sur le cancer
- Cyrille Cuenin🇫🇷Centre international de recherche sur le cancer
- Carlotta Sacerdote🇮🇹Italian institute for Genomic Medicine; Piedmont Reference Center for Epidemiology and Cancer Prevention
- Silvia Polidoro🇮🇹Italian institute for Genomic Medicine
- Mazda Jenab🇫🇷Centre international de recherche sur le cancer
- Pierre L. Hainaut🇫🇷Centre international de recherche sur le cancer
- Paolo Vineis · autor de correspondencia
- Zdenko Herceg🇬🇧🇫🇷 · autor de correspondenciaNIHR Imperial Biomedical Research Centre; Centre international de recherche sur le cancer; Imperial College London
Resumen
El texto original del estudio, en el idioma en que se publicó.
Changes in DNA methylation patterns are a hallmark of tobacco-induced carcinogenesis. We have conducted a randomized 4-week intervention trial to investigate the effects of three dietary regimens to modify DNA methylation patterns in peripheral white blood cells of heavy smokers. A group of 88 smokers were randomly assigned to and distributed among three diets, including (1) normal isocaloric diet (balanced in fruits and vegetables), according to international guidelines; (2) a diet enriched in flavonoids and isothiocyanates (particularly cruciferous vegetables); (3) a regimen consisting of diet 1 supplemented with flavonoids (green tea and soy products). Methylation patterns were analyzed by pyrosequencing in LINE1 (Long Interspersed DNA Elements), RASSF1A, ARF and CDKN2a (tumor suppressor genes), MLH1 (mismatch DNA repair) and MTHFR (folate metabolism). Three distinct patterns of methylation were observed. In LINE1, methylation showed a small but reproducible increase with all three regimens. MTHFR was constitutively methylated with no significant modulation by diets. The four other loci showed low basal levels of methylation with no substantial change after intervention. These data suggest that the isocaloric diet may stabilize global epigenetic (LINE1 DNA methylation) patterns in peripheral white blood cells but does not provide evidence for methylation changes in specific genes associated with this short-term dietary intervention.
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