Modulation of mouse gastrointestinal motility by allyl isothiocyanate, a constituent of cruciferous vegetables (Brassicaceae): evidence for TRPA1-independent effects
Capasso R, Aviello G, Romano B, Borrelli F, De Petrocellis L, Di Marzo V, Izzo AA
British journal of pharmacology · 38 citas
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Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Animales
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2011-09-28 · Br J Pharmacol · vol. 165 · número 6 · págs. 1966–1977
- Editorial
- Wiley
- Citado
- 51 citas · más que el 87% de estudios similares · 2.5× el promedio de su campo
- Referencias
- 49 trabajos
- Acceso
- Lectura gratuita
- Áreas de investigación
- Ion Channels and Receptors · Phytochemicals and Antioxidant Activities · Postharvest Quality and Shelf Life Management
- Palabras clave
- Allyl isothiocyanate, Chemistry, Motility, Pharmacology, Contractility, Ryanodine receptor, In vivo, Thapsigargin, Biochemistry, Biology, Intracellular, Endocrinology, Cell biology
- MeSH
- colon, ileum, animals, mice, inbred icr, humans, mice, brassicaceae, vegetables, isothiocyanates, parasympatholytics, gastrointestinal motility, muscle contraction, male, transient receptor potential channels, hek293 cells, in vitro techniques, trpa1 cation channel
7 autores
- Raffaele Capasso🇮🇹University of Naples Federico II
- Gabriella Aviello🇮🇹University of Naples Federico II
- Barbara Romano🇮🇹University of Naples Federico II
- Francesca Borrelli🇮🇹University of Naples Federico II
- Luciano De Petrocellis🇮🇹National Research Council
- Vincenzo Di Marzo🇮🇹 · autor de correspondenciaNational Research Council
- Angelo Antonio Izzo🇮🇹 · autor de correspondenciaUniversity of Naples Federico II
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background and purpose
Allyl isothiocyanate (AITC, mustard oil), a constituent of many common cruciferous vegetables (Brassicaceae), activates transient receptor potential of ankyrin type-1 (TRPA1) channels, claimed to regulate gastrointestinal contractility. In this study, we have investigated the effect of AITC on intestinal motility.
Experimental approach
Effects of AITC were investigated in vivo on upper gastrointestinal transit in mice and in mouse isolated ileum [contractions induced by electrical field stimulation (EFS), acetylcholine and spontaneous contractility]. The contractor activity of AITC was studied in mouse isolated colon. The ability of TRPA1 channel antagonists to block AITC-induced elevation of intracellular Ca(2+) [Ca(2+)](i) was assessed in HEK293 cells transfected with rat TRPA1 channels.
Key results
AITC increased [Ca(2+)](i) in HEK293 cells, reduced ileal contractility (acetylcholine-, EFS-induced contractions and spontaneous contractility), but contracted the isolated colon. Gentamicin and camphor (non-selective TRPA1 channel antagonists), HC-030031 and AP18 (selective TRPA1 channel agonists) inhibited AITC-induced effects in HEK293 cells but not in the ileum or colon. AITC-induced contractions were reduced by tetrodotoxin and strongly reduced by nifedipine, cyclopiazonic acid and ryanodine. In vivo, AITC reduced (following i.p. administration) or increased (following intragastric administration) upper gastrointestinal transit in mice These effects were not affected by HC-030031.
Conclusion and implications
AITC, depending, in vitro, on the regions of gut examined and, in vivo, on the route of administration, exerted both stimulatory and inhibitory effects on intestinal motility, which were not sensitive to TRPA1 channel antagonists. The proposition that TRPA1 channels are the primary targets for AITC to induce contraction should be revised.
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