Inhibitory effect of blueberry polyphenolic compounds on oleic acid-induced hepatic steatosis in vitro
Liu Y, Wang D, Zhang D, Lv Y, Wei Y, Wu W, Zhou F, Tang M, Mao T, Li M, Ji B
Journal of agricultural and food chemistry · 60 citas
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- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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Publicación
- Publicado
- 2011-10-15 · J Agric Food Chem · vol. 59 · número 22 · págs. 12254–12263
- Editorial
- American Chemical Society
- Citado
- 87 citas · más que el 93% de estudios similares · 3.9× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 45 trabajos
- Acceso
- De pago
- Áreas de investigación
- Liver Disease Diagnosis and Treatment · Diet, Metabolism, and Disease · Phytochemicals and Antioxidant Activities
- Palabras clave
- Caffeic acid, Steatosis, Chemistry, Polyphenol, Nonalcoholic fatty liver disease, Chlorogenic acid, Fatty liver, Oleic acid, Triglyceride, Insulin resistance, Biochemistry, Food science, Internal medicine, Insulin, Antioxidant, Medicine, Cholesterol
- MeSH
- humans, fatty liver, oleic acid, triglycerides, plant extracts, protective agents, models, biological, hep g2 cells, polyphenols, non-alcoholic fatty liver disease, blueberry plants
11 autores
- Yixiang Liu🇨🇳China Agricultural University
- Dan Wang🇨🇳China Agricultural University
- Di Zhang🇨🇳China Agricultural University
- Yechun Lv🇨🇳China Agricultural University
- Ying Wei🇨🇳China Agricultural University
- Wei Wu🇨🇳China Agricultural University
- Feng Li Zhou🇨🇳China Agricultural University
- Miaomiao Tang🇨🇳China Agricultural University
- Ting Mao🇨🇳China Agricultural University
- Mengmeng Li🇨🇳China Agricultural University
- Baoping Ji🇨🇳 · autor de correspondenciaChina Agricultural University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Nonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases worldwide and is closely associated with metabolic syndromes, such as obesity, diabetes, and insulin resistance. Nonalcoholic fatty liver (NAFL), also called simple steatosis, is the initial phase of NAFLD, which is accompanied the characteristic pathological overaccumulation of lipids without inflammation. To prevent NAFLD from reaching the NAFL stage through dietary therapy, in the present work, wild Chinese blueberries (Vacciniun spp.) were selected for their well-known benefits in inhibiting metabolic syndrome. After being purified from wild Chinese blueberries, polyphenol-rich extracts were subsequently separated into three fractions, namely, anthocyanin-rich fraction, phenolic acid-rich fraction, and ethyl acetate extract. The inhibition of oleic acid (OA)-induced triglyceride (TG) deposition in HepG 2 cells was referred to as the potential activity of preventing NAFL. Biochemical indicators, such as cytotoxicity, TG level, levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and intracellular reactive oxygen species, were used to evaluate the analogous pathological stage of NAFLD. The results show that OA ≤ 1.0 mM exhibits a dose-dependent induction of TG accumulation, and no inflammation was observed based on the changes in ALT and AST levels. Therefore, 1.0 mM OA was used to simulate an in vitro fatty liver. Blueberry polyphenol-rich extract efficiently inhibited OA-induced TG accumulation in HepG2 cells, and the phenolic acid-rich fraction performed efficiently. Seven phenolic acids were subsequently identified using a high-performance liquid chromatography assay, and the main types were caffeic, chlorogenic, ferulic, p-coumaric, and cinnamic acids. These phenolic acid standards also displayed good efficiency in inhibiting TG accumulation in HepG2 cells. These results imply that wild Chinese blueberries have a potential preventive effect on NAFLD in its early stage, and phenolic acids are the most efficient component.
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