Water extract from the leaves of Withania somnifera protect RA differentiated C6 and IMR-32 cells against glutamate-induced excitotoxicity
Kataria H, Wadhwa R, Kaul SC, Kaur G
PloS one · 46 citas
Etiquetas de revisión
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Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institute of Advanced Industrial Science and Technology
- Gobierno
- Department of Biotechnology, Ministry of Science and Technology, India
- Gobierno
- Council of Scientific and Industrial Research, India
Según 3 financiadores declarados.
Publicación
- Publicado
- 2012-05-14 · PLoS One · vol. 7 · número 5 · pág. e37080
- Editorial
- Public Library of Science
- Citado
- 77 citas · más que el 99% de estudios similares · 19.8× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 86 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Neuroscience and Neural Engineering
- Palabras clave
- Glutamate receptor, Withania somnifera, Neuroprotection, Neurotoxicity, Excitotoxicity, Programmed cell death, Viability assay, Biology, Neurodegeneration, Cell biology, Neural cell adhesion molecule, Pharmacology, Biochemistry, Chemistry, Medicine, Cell, Apoptosis, Cell adhesion, Toxicity, Internal medicine, Pathology
- MeSH
- neurons, cell line, animals, humans, rats, withania, plant leaves, plants, medicinal, nerve degeneration, tretinoin, glial fibrillary acidic protein, neurofilament proteins, glutamic acid, neural cell adhesion molecules, excitatory amino acid antagonists, neuroprotective agents, plant extracts, neurotoxins, cell death, cell differentiation, hsp70 heat-shock proteins, matrix metalloproteinase 2, matrix metalloproteinase 9
4 autores
- Hardeep Kataria🇮🇳Guru Nanak Dev University
- Renu Wadhwa🇯🇵 · autor de correspondenciaNational Institute of Advanced Industrial Science and Technology
- Sunil C. Kaul🇯🇵National Institute of Advanced Industrial Science and Technology
- Gurcharan Kaur🇮🇳 · autor de correspondenciaGuru Nanak Dev University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Glutamate neurotoxicity has been implicated in stroke, head trauma, multiple sclerosis and neurodegenerative disorders. Search for herbal remedies that may possibly act as therapeutic agents is an active area of research to combat these diseases. The present study was designed to investigate the neuroprotective role of Withania somnifera (Ashwagandha), also known as Indian ginseng, against glutamate induced toxicity in the retinoic acid differentiated rat glioma (C6) and human neuroblastoma (IMR-32) cells. The neuroprotective activity of the Ashwagandha leaves derived water extract (ASH-WEX) was evaluated. Cell viability and the expression of glial and neuronal cell differentiation markers was examined in glutamate challenged differentiated cells with and without the presence of ASH-WEX. We demonstrate that RA-differentiated C6 and IMR-32 cells, when exposed to glutamate, undergo loss of neural network and cell death that was accompanied by increase in the stress protein HSP70. ASH-WEX pre-treatment inhibited glutamate-induced cell death and was able to revert glutamate-induced changes in HSP70 to a large extent. Furthermore, the analysis on the neuronal plasticity marker NCAM (Neural cell adhesion molecule) and its polysialylated form, PSA-NCAM revealed that ASH-WEX has therapeutic potential for prevention of neurodegeneration associated with glutamate-induced excitotoxicty.
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