Isothiocyanate concentrations and interconversion of sulforaphane to erucin in human subjects after consumption of commercial frozen broccoli compared to fresh broccoli
Saha S, Hollands W, Teucher B, Needs PW, Narbad A, Ortori CA, Barrett DA, Rossiter JT, Mithen RF, Kroon PA
Molecular nutrition & food research · 94 citas
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Sin resultado de salud
- Consumo medido con
- Comida provista por los investigadores
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- Biotechnology and Biological Sciences Research Council
- Subvenciones
- Biotechnology and Biological Sciences Research Council (42/D 20475); Biotechnology and Biological Sciences Research Council (BBS/E/F/00044431); Biotechnology and Biological Sciences Research Council (BBS/E/F/00044434); Biotechnology and Biological Sciences Research Council (BBS/E/F/00044453); Biotechnology and Biological Sciences Research Council (42/D1800); Biotechnology and Biological Sciences Research Council (BBS/E/F/00044453); Biotechnology and Biological Sciences Research Council (BBS/E/F/00041700); Biotechnology and Biological Sciences Research Council (BBS/E/F/00044431); Biotechnology and Biological Sciences Research Council (BBS/E/F/00044434)
Según 1 financiador declarado.
Publicación
- Publicado
- 2012-10-27 · Mol Nutr Food Res · vol. 56 · número 12 · págs. 1906–1916
- Editorial
- Wiley
- Citado
- 127 citas · más que el 95% de estudios similares · 4.0× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 24 trabajos
- Acceso
- De pago
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Phytochemicals and Antioxidant Activities · Garlic and Onion Studies
- Palabras clave
- Sulforaphane, Glucoraphanin, Myrosinase, Blanching, Chemistry, Bioavailability, Food science, Glucosinolate, Sinigrin, Asparagus, Isothiocyanate, Mercapturic acid, Urine, Brassica, Brassica oleracea, Biochemistry, Cysteine, Botany, Pharmacology, Enzyme, Medicine, Biology
- MeSH
- gastrointestinal tract, humans, brassica, sulfides, oximes, thiocyanates, imidoesters, isothiocyanates, sulfoxides, glycoside hydrolases, glucosinolates, anticarcinogenic agents, cross-over studies, biological availability, kinetics, freezing, food handling, adult, aged, middle aged, female, male, young adult, metagenome
10 autores
- Shikha Saha🇬🇧Norwich Research Park; Quadram Institute
- Wendy J. Hollands🇬🇧Norwich Research Park; Quadram Institute
- Birgit Teucher🇬🇧Norwich Research Park; Quadram Institute
- Paul W. Needs🇬🇧Norwich Research Park; Quadram Institute
- Arjan Narbad🇬🇧Norwich Research Park; Quadram Institute
- Catharine A. Ortori🇬🇧University of Nottingham
- David A. Barrett🇬🇧University of Nottingham
- John Trevor Rossiter🇬🇧Imperial College London
- Richard F. Mithen🇬🇧Norwich Research Park; Quadram Institute
- Paul Antony Kroon🇬🇧 · autor de correspondenciaNorwich Research Park; Quadram Institute
Resumen
El texto original del estudio, en el idioma en que se publicó.
Scope
Sulforaphane (a potent anticarcinogenic isothiocyanate derived from glucoraphanin) is widely considered responsible for the protective effects of broccoli consumption. Broccoli is typically purchased fresh or frozen and cooked before consumption. We compared the bioavailability and metabolism of sulforaphane from portions of lightly cooked fresh or frozen broccoli, and investigated the bioconversion of sulforaphane to erucin.
Methods and results
Eighteen healthy volunteers consumed broccoli soups produced from fresh or frozen broccoli florets that had been lightly cooked and sulforaphane thio-conjugates quantified in plasma and urine. Sulforaphane bioavailability was about tenfold higher for the soups made from fresh compared to frozen broccoli, and the reduction was shown to be due to destruction of myrosinase activity by the commercial blanching-freezing process. Sulforaphane appeared in plasma and urine in its free form and as several thio-conjugates forms. Erucin N-acetyl-cysteine conjugate was a significant urinary metabolite, and it was shown that human gut microflora can produce sulforaphane, erucin, and their nitriles from glucoraphanin.
Conclusion
The short period of blanching used to produce commercial frozen broccoli destroys myrosinase and substantially reduces sulforaphane bioavailability. Sulforaphane was converted to erucin and excreted in urine, and it was shown that human colonic flora were capable of this conversion.
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