Pterostilbene, a bioactive component of blueberries, suppresses the generation of breast cancer stem cells within tumor microenvironment and metastasis via modulating NF-κB/microRNA 448 circuit
Mak KK, Wu AT, Lee WH, Chang TC, Chiou JF, Wang LS, Wu CH, Huang CY, Shieh YS, Chao TY, Ho CT, Yen GC, Yeh CT
Molecular nutrition & food research · 82 citas
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Eventos clínicos como enfermedad o muerte
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- Ministry of Education, Taiwan
- Gobierno
- NSC
- Universidad u hospital
- Center of Excellence for Cancer Research at Taipei Medical University
Según 3 financiadores declarados.
Publicación
- Publicado
- 2013-03-15 · Mol Nutr Food Res · vol. 57 · número 7 · págs. 1123–1134
- Editorial
- Wiley
- Citado
- 112 citas · más que el 93% de estudios similares · 3.9× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 35 trabajos
- Acceso
- De pago
- Áreas de investigación
- Sirtuins and Resveratrol in Medicine · Cancer Cells and Metastasis · Immune cells in cancer
- Palabras clave
- Pterostilbene, Cancer research, Metastasis, Carcinogenesis, Cancer stem cell, Cancer, Downregulation and upregulation, Chemistry, microRNA, NF-κB, Tumor microenvironment, Breast cancer, Biology, Pharmacology, Signal transduction, Medicine, Resveratrol, Internal medicine, Biochemistry, Tumor cells
- MeSH
- cell line, tumor, animals, humans, mice, mice, scid, breast neoplasms, stilbenes, vimentin, nf-kappa b, cadherins, nuclear proteins, micrornas, antigens, cd, down-regulation, gene silencing, female, beta catenin, hypoxia-inducible factor 1, alpha subunit, neoplastic stem cells, epithelial-mesenchymal transition, tumor microenvironment, mcf-7 cells, carcinogenesis, blueberry plants, twist-related protein 1, hyaluronan receptors
13 autores
- Ka‐Kit Mak🇹🇼Taipei Medical University
- Alexander T.H. Wu🇹🇼Taipei Medical University Hospital; Taipei Medical University
- Wei‐Hwa Lee🇹🇼Taipei Medical University-Shuang Ho Hospital
- Tung‐Cheng Chang🇹🇼Taipei Medical University-Shuang Ho Hospital; Taipei Medical University
- Jeng‐Fong Chiou🇹🇼Taipei Medical University Hospital; Taipei Medical University
- L.S. Wang🇹🇼Taipei Medical University-Shuang Ho Hospital; Taipei Medical University
- Chih‐Hsiung Wu🇹🇼Taipei Medical University-Shuang Ho Hospital
- Chi‐Ying F. Huang🇹🇼National Yang Ming Chiao Tung University; National Yang Ming University Hospital; National Yang Ming Chiao Tung University
- Yi‐Shing Shieh🇹🇼Tri-Service General Hospital; National Defense Medical Center
- Tsu‐Yi Chao🇹🇼Taipei Medical University-Shuang Ho Hospital; Taipei Medical University
- Chi‐Tang Ho🇺🇸Rutgers, The State University of New Jersey
- Gow‐Chin Yen🇹🇼 · autor de correspondenciaNational Chung Hsing University
- Chi‐Tai Yeh🇹🇼 · autor de correspondenciaTaipei Medical University-Shuang Ho Hospital; Taipei Medical University; National Defense Medical Center
Resumen
El texto original del estudio, en el idioma en que se publicó.
Scope
Tumor-associated macrophages (TAMs) have been shown to promote metastasis and malignancy. Pterostilbene, a natural stilbene isolated from blueberries, has been suggested for anti-cancer effects. Here, we explored the potential cancer stem cells (CSCs)/TAM modulating effects of pterostilbene in breast cancer.
Methods and results
Using flowcytometric and Boyden chamber assay, we showed MCF7 and MDA-MB-231 cells cocultured with M2 TAMs exhibited increased percentage of CD44(+) /CD24(-) CSC population and migratory/invasive abilities. RT-PCR results showed that CD44(+) /CD24(-) cells expressed an increased level of HIF-1α, β-catenin, Twist1, and NF-κB and enhanced tumor sphere forming ability. Additionally, pterostilbene treatment dose dependently overcame M2 TAM-induced enrichment of CSCs and metastatic potential of breast cancer cells. Mechanistically, pterostilbene suppressed NFκB, Twist1, vimentin, and increased E-cadherin expression. Using siRNA technique, we demonstrated that pterostilbene-mediated NFκB downregulation was correlated to an increased amount of microRNA 448. Finally, pterostilbene-mediated suppression in tumorigenesis and metastasis was validated by noninvasive bioluminescence in mice bearing M2 TAM cocultured MDA-MB-231 tumor.
Conclusion
Pterostilbene effectively suppresses the generation of CSCs and metastatic potential under the influence of M2 TAMs via modulating EMT associated signaling pathways, specifically NF-κB/miR488 circuit. Thus, pterostilbene could be an ideal anti-CSC agent in clinical settings.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.