Influence of vitamin D status and vitamin D3 supplementation on genome wide expression of white blood cells: a randomized double-blind clinical trial
Hossein-nezhad A, Spira A, Holick MF
PloS one · 181 citas
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institutes of Health
- Gobierno
- National Center for Research Resources
- Gobierno
- NCRR NIH HHS
- Subvenciones
- National Center for Research Resources (UL-1-RR-25711)
Según 3 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2013-03-20 · PLoS One · vol. 8 · número 3 · pág. e58725
- Editorial
- Public Library of Science
- Citado
- 293 citas · más que el 100% de estudios similares · 19.6× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 33 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Vitamin D Research Studies · Nutrition and Health Studies · Nutrition, Genetics, and Disease
- Palabras clave
- vitamin D status, vitamin D3 supplementation, 25-hydroxyvitamin D, vitamin D deficiency, gene expression, leukocytes, buffy coat, vitamin D response element, TRIM27, CD83, COPB2, immune function, DNA repair, stress response
- MeSH
- leukocytes, humans, vitamin d deficiency, cholecalciferol, vitamin d, oligonucleotide array sequence analysis, double-blind method, gene expression, vitamin d response element, dose-response relationship, drug, adult, female, male, young adult
3 autores
- Arash Hossein‐Nezhad🇺🇸Boston University; Boston Medical Center
- Avrum E. Spira🇺🇸Boston University; Boston Medical Center
- Michael F. Holick🇺🇸 · autor de correspondenciaBoston University; Boston Medical Center
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Although there have been numerous observations of vitamin D deficiency and its links to chronic diseases, no studies have reported on how vitamin D status and vitamin D3 supplementation affects broad gene expression in humans. The objective of this study was to determine the effect of vitamin D status and subsequent vitamin D supplementation on broad gene expression in healthy adults. (Trial registration: ClinicalTrials.gov NCT01696409).
Methods and findings
A randomized, double-blind, single center pilot trial was conducted for comparing vitamin D supplementation with either 400 IUs (n = 3) or 2000 IUs (n = 5) vitamin D3 daily for 2 months on broad gene expression in the white blood cells collected from 8 healthy adults in the winter. Microarrays of the 16 buffy coats from eight subjects passed the quality control filters and normalized with the RMA method. Vitamin D3 supplementation that improved serum 25-hydroxyvitamin D concentrations was associated with at least a 1.5 fold alteration in the expression of 291 genes. There was a significant difference in the expression of 66 genes between subjects at baseline with vitamin D deficiency (25(OH)D20 ng/ml. After vitamin D3 supplementation gene expression of these 66 genes was similar for both groups. Seventeen vitamin D-regulated genes with new candidate vitamin D response elements including TRIM27, CD83, COPB2, YRNA and CETN3 which have been shown to be important for transcriptional regulation, immune function, response to stress and DNA repair were identified.
Conclusion/significance
Our data suggest that any improvement in vitamin D status will significantly affect expression of genes that have a wide variety of biologic functions of more than 160 pathways linked to cancer, autoimmune disorders and cardiovascular disease with have been associated with vitamin D deficiency. This study reveals for the first time molecular finger prints that help explain the nonskeletal health benefits of vitamin D.
Trial registration
ClinicalTrials.gov NCT01696409.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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