No independent, but an interactive, role of calcium-activated potassium channels in human cutaneous active vasodilation
Brunt VE, Fujii N, Minson CT
Journal of applied physiology (Bethesda, Md. : 1985) · 39 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Ensayo clínico controlado (clasificado por nuestro filtro de IA)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Heart, Lung, and Blood Institute
- Gobierno
- NHLBI NIH HHS
- Subvenciones
- National Heart, Lung, and Blood Institute (HL081671)
Según 2 financiadores declarados.
Publicación
- Publicado
- 2013-08-23 · J Appl Physiol (1985) · vol. 115 · número 9 · págs. 1290–1296
- Editorial
- American Physiological Society
- Citado
- 46 citas · más que el 92% de estudios similares · 3.2× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 51 trabajos
- Acceso
- Acceso abierto (copia en repositorio)
- Áreas de investigación
- Thermoregulation and physiological responses · Heart Rate Variability and Autonomic Control · Circadian rhythm and melatonin
- Palabras clave
- Vasodilation, Tetraethylammonium chloride, Chemistry, Tetraethylammonium, Microdialysis, Potassium channel, Nitric oxide, Potassium, Calcium-activated potassium channel, Nitric oxide synthase, Calcium, Anesthesia, Internal medicine, Pharmacology, Endocrinology, Medicine, Biochemistry, Extracellular
- MeSH
- skin, humans, nitric oxide, tetraethylammonium, ng-nitroarginine methyl ester, potassium channels, calcium-activated, biological factors, laser-doppler flowmetry, microdialysis, regional blood flow, vasodilation, adult, female, male, nitric oxide synthase, hot temperature, young adult
3 autores
- Vienna Elizabeth Brunt🇺🇸University of Oregon
- Naoto Fujii🇺🇸University of Oregon
- Christopher Todd Minson🇺🇸 · autor de correspondenciaUniversity of Oregon
Resumen
El texto original del estudio, en el idioma en que se publicó.
In human cutaneous microvasculature, endothelium-derived hyperpolarizing factors (EDHFs) account for a large portion of vasodilation associated with local stimuli. Thus we sought to determine the role of EDHFs in active vasodilation (AVD) to passive heating in two protocols. Whole body heating was achieved using water-perfused suits (core temperature increase of 0.8-1.0°C), and skin blood flow was measured using laser-Doppler flowmetry. In the first protocol, four sites were perfused continuously via microdialysis with: 1) control; 2) tetraethylammonium (TEA) to block calcium-activated potassium (KCa) channels, and thus the actions of EDHFs; 3) N-nitro-l-arginine methyl ester (l-NAME) to inhibit nitric oxide synthase (NOS); and 4) TEA + l-NAME (n = 8). Data are presented as percent maximal cutaneous vascular conductance (CVC). TEA had no effect on AVD (CVC during heated plateau: control 57.4 ± 4.9% vs. TEA 63.2 ± 5.2%, P = 0.27), indicating EDHFs are not obligatory. l-NAME attenuated plateau CVC to 33.7 ± 5.4% (P < 0.01 vs. control); while TEA + l-NAME augmented plateau CVC compared with l-NAME alone (49.7 ± 5.3%, P = 0.02). From these data, it appears combined blockade of EDHFs and NOS necessitates dilation through other means, possibly through inward rectifier (KIR) and/or ATP-sensitive (KATP) potassium channels. To test this second hypothesis, we measured AVD at the following sites (n = 8): 1) control, 2) l-NAME, 3) l-NAME + TEA, and 4) l-NAME + TEA + barium chloride (BaCl2; KIR and KATP blocker). The addition of BaCl2 to l-NAME + TEA reduced plateau CVC to 32.7 ± 6.6% (P = 0.02 vs. l-NAME + TEA), which did not differ from the l-NAME site. These data combined demonstrate a complex interplay between vasodilatory pathways, with cross-talk between NO, KCa channels, and KIR and/or KATP channels.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.