Water extract of Ashwagandha leaves has anticancer activity: identification of an active component and its mechanism of action
Wadhwa R, Singh R, Gao R, Shah N, Widodo N, Nakamoto T, Ishida Y, Terao K, Kaul SC
PloS one · 43 citas
Etiquetas de revisión
Vínculos de autores con la industriaSolo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institute of Advanced Industrial Science and Technology
- Gobierno
- Ministry of Education, Culture, Sports, Science and Technology
- Autores
- Al menos un autor declara un vínculo económico con la industria
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2013-10-10 · PLoS One · vol. 8 · número 10 · pág. e77189
- Editorial
- Public Library of Science
- Citado
- 89 citas · más que el 99% de estudios similares · 20.5× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 51 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Plant-Derived Bioactive Compounds · Food Science and Nutritional Studies
- Palabras clave
- Cyclin D1, In vivo, Cancer cell, Cell cycle checkpoint, Metastasis, Pharmacology, Cell cycle, Chemistry, Cancer research, Biology, Cancer, Traditional medicine, Apoptosis, Biochemistry, Medicine, Biotechnology
- MeSH
- cell line, tumor, animals, humans, mice, withania, plant leaves, water, plant extracts, antineoplastic agents, solvents, xenograft model antitumor assays, cell proliferation, female
9 autores
- Renu Wadhwa🇯🇵National Institute of Advanced Industrial Science and Technology
- Rumani Singh🇯🇵National Institute of Advanced Industrial Science and Technology
- Ran Gao🇯🇵National Institute of Advanced Industrial Science and Technology
- Navjot Shah🇯🇵National Institute of Advanced Industrial Science and Technology
- Nashi Widodo🇯🇵National Institute of Advanced Industrial Science and Technology
- Tomoko Nakamoto🇯🇵National Institute of Advanced Industrial Science and Technology
- Yoshiyuki Ishida🇮🇩University of Brawijaya
- Keiji Terao🇮🇩University of Brawijaya
- Sunil C. Kaul🇯🇵 · autor de correspondenciaNational Institute of Advanced Industrial Science and Technology
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Cancer is a leading cause of death accounting for 15-20% of global mortality. Although advancements in diagnostic and therapeutic technologies have improved cancer survival statistics, 75% of the world population live in underdeveloped regions and have poor access to the advanced medical remedies. Natural therapies hence become an alternative choice of treatment. Ashwagandha, a tropical herb used in Indian Ayurvedic medicine, has a long history of its health promoting and therapeutic effects. In the present study, we have investigated an anticancer activity in the water extract of Ashwagandha leaves (ASH-WEX).
Methodology/principal findings
Anticancer activity in the water extract of Ashwagandha leaves (ASH-WEX) was detected by in vitro and in vivo assays. Bioactivity-based size fractionation and NMR analysis were performed to identify the active anticancer component(s). Mechanism of anticancer activity in the extract and its purified component was investigated by biochemical assays. We report that the ASH-WEX is cytotoxic to cancer cells selectively, and causes tumor suppression in vivo. Its active anticancer component was identified as triethylene glycol (TEG). Molecular analysis revealed activation of tumor suppressor proteins p53 and pRB by ASH-WEX and TEG in cancer cells. In contrast to the hypophosphorylation of pRB, decrease in cyclin B1 and increase in cyclin D1 in ASH-WEX and TEG-treated cancer cells (undergoing growth arrest), normal cells showed increase in pRB phosphorylation and cyclin B1, and decrease in cyclin D1 (signifying their cell cycle progression). We also found that the MMP-3 and MMP-9 that regulate metastasis were down regulated in ASH-WEX and TEG-treated cancer cells; normal cells remained unaffected.
Conclusion
We provide the first molecular evidence that the ASH-WEX and TEG have selective cancer cell growth arrest activity and hence may offer natural and economic resources for anticancer medicine.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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