Effects of low-level laser therapy on M1-related cytokine expression in monocytes via histone modification
Chen CH, Wang CZ, Wang YH, Liao WT, Chen YJ, Kuo CH, Kuo HF, Hung CH
Mediators of inflammation · 35 citas
Etiquetas de revisión
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Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- Kaohsiung Municipal Ta-Tung Hospital
- Gobierno
- National Institute for Health and Care Research
- Gobierno
- National Science Council
- Gobierno
- National Health Research Institutes
- Gobierno
- National Institute for Health Research (NIHR)
- Subvenciones
- National Institute for Health and Care Research (EX102-9914EC); National Health Research Institutes (NSC 101-); Kaohsiung Municipal Ta-Tung Hospital (NSC 101-2314-B-037 -002); National Science Council (KMTTH-101-012); Kaohsiung Municipal Ta-Tung Hospital (KMTTH-101-012); National Health Research Institutes (NHRI-EX102); National Science Council (EX102-9914EC); National Science Council (NSC 101-2314-B-037 -002); Kaohsiung Municipal Ta-Tung Hospital (EX102-9914EC); National Institute for Health and Care Research (NSC 101-2314-B-037 -002); National Institute for Health and Care Research (KMTTH-101-012)
Según 5 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2014-01-01 · Mediators Inflamm · vol. 2014 · págs. 1–13
- Editorial
- Hindawi Publishing Corporation
- Citado
- 62 citas · más que el 94% de estudios similares · 4.1× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 37 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Laser Applications in Dentistry and Medicine · Salivary Gland Disorders and Functions · Exercise and Physiological Responses
- Palabras clave
- CXCL10, Chemokine, Cytokine, Chromatin immunoprecipitation, Monocyte, Cancer research, Flow cytometry, CCL2, Molecular biology, Chemistry, Immunology, Biology, Gene expression, Immune system, Promoter, Gene
- MeSH
- monocytes, cell line, chromatin, mitochondria, humans, inflammation, tumor necrosis factor-alpha, histones, chemokines, cytokines, lasers, gene expression regulation, chemokine ccl2, chemokine cxcl10, promoter regions, genetic, low-level light therapy
8 autores
- Chia‐Hsin Chen🇹🇼Kaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital; Kaohsiung Municipal Ta-Tung Hospital
- Chau‐Zen Wang🇹🇼Kaohsiung Medical University
- Yan‐Hsiung Wang🇹🇼Kaohsiung Medical University
- Wei‐Ting Liao🇹🇼Kaohsiung Medical University
- Yi‐Jen Chen🇹🇼Kaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
- Chang‐Hung Kuo🇹🇼Kaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
- Hsuan‐Fu Kuo🇹🇼Kaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital; Kaohsiung Municipal Ta-Tung Hospital
- Chih‐Hsing Hung🇹🇼 · autor de correspondenciaKaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital; Kaohsiung Municipal Hsiao-Kang Hospital
Resumen
El texto original del estudio, en el idioma en que se publicó.
Low-level laser therapy (LLLT) has been used in the treatment of radiotherapy-induced oral mucositis and allergic rhinitis. However, the effects of LLLT on human monocyte polarization into M1 macrophages are unknown. To evaluate the effects of LLLT on M1-related cytokine and chemokine production and elucidate the mechanism, the human monocyte cell line THP-1 was treated with different doses of LLLT. The expression of M1-related cytokines and chemokines (CCL2, CXCL10, and TNF-α) was determined by ELISA and real-time PCR. LLLT-associated histone modifications were examined by chromatin immunoprecipitation (ChIP) assays. Mitochondrial involvement in the LLLT-induced M1-related cytokine expression was evaluated by quantitative real-time PCR. Flow cytometry was used to detect the cell surface markers for monocyte polarization. The results showed that LLLT (660 nm) significantly enhanced M1-related cytokine and chemokine expression in mRNA and protein levels. Mitochondrial copy number and mRNA levels of complex I-V protein were increased by LLLT (1 J/cm(2)). Activation of M1 polarization was concomitant with histone modification at TNF-α gene locus and IP-10 gene promoter area. This study indicates that LLLT (660 nm) enhanced M1-related cytokine and chemokine expression via mitochondrial biogenesis and histone modification, which may be a potent immune-enhancing agent for the treatment of allergic diseases.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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