β-Amyloid1-42, HIV-1Ba-L (clade B) infection and drugs of abuse induced degeneration in human neuronal cells and protective effects of ashwagandha (Withania somnifera) and its constituent Withanolide A
Kurapati KR, Samikkannu T, Atluri VS, Kaftanovskaya E, Yndart A, Nair MP
PloS one · 24 citas
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- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institute of Mental Health
- Gobierno
- National Institute on Drug Abuse
- Gobierno
- NIMH NIH HHS
- Gobierno
- NIDA NIH HHS
- Subvenciones
- National Institute on Drug Abuse (1R01-DA027049); National Institute on Drug Abuse (R01DA021537); National Institute on Drug Abuse (R37DA025576); National Institute on Drug Abuse (1R037DA025576); National Institute of Mental Health (R01 MH085259); National Institute on Drug Abuse (5R01DA021537); National Institute of Mental Health (1R01MH085259); National Institute on Drug Abuse (R01-DA027049)
Según 4 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2014-11-21 · PLoS One · vol. 9 · número 11 · pág. e112818
- Editorial
- Public Library of Science
- Citado
- 37 citas · más que el 98% de estudios similares · 12.2× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 58 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection · Alzheimer's disease research and treatments
- Palabras clave
- Withania somnifera, Pharmacology, Neuroprotection, MTT assay, Toxicity, Chemistry, Biology, Medicine, Biochemistry, Pathology, Internal medicine, Cell
- MeSH
- neurons, cell line, humans, hiv-1, withania, peptide fragments, neuroprotective agents, withanolides, amyloid beta-peptides, illicit drugs
6 autores
- Kesava Rao Venkata Kurapati🇺🇸Florida International University
- Thangavel Samikkannu🇺🇸Florida International University
- Venkata Subba Rao Atluri🇺🇸Florida International University
- Elena M. Kaftanovskaya🇺🇸Florida International University
- Adriana Yndart🇺🇸Florida International University
- Madhavan Nair🇺🇸 · autor de correspondenciaFlorida International University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Alzheimer's disease (AD) is characterized by progressive dysfunction of memory and higher cognitive functions with abnormal accumulation of extracellular amyloid plaques and intracellular neurofibrillary tangles throughout cortical and limbic brain regions. Withania somnifera (WS) also known as 'ashwagandha' (ASH) is used widely in Ayurvedic medicine as a nerve tonic and memory enhancer. However, there is paucity of data on potential neuroprotective effects of ASH against β-Amyloid (1-42) (Aβ) induced neuropathogenesis. In the present study, we have tested the neuroprotective effects of Methanol: Chloroform (3:1) extract of ASH and its constituent Withanolide A (WA) against Aβ induced toxicity, HIV-1(Ba-L) (clade B) infection and the effects of drugs of abuse using a human neuronal SK-N-MC cell line. Aβ when tested individually, induced cytotoxic effects in SK-N-MC cells as shown by increased trypan blue stained cells. However, when ASH was added to Aβ treated cells the toxic effects were neutralized. This observation was supported by cellular localization of Aβ, MTT formazan exocytosis, and the levels of acetylcholinesterase activity, confirming the chemopreventive or protective effects of ASH against Aβ induced toxicity. Further, the levels of MAP2 were significantly increased in cells infected with HIV-1(Ba-L) (clade B) as well as in cells treated with Cocaine (COC) and Methamphetamine (METH) compared with control cells. In ASH treated cells the MAP2 levels were significantly less compared to controls. Similar results were observed in combination experiments. Also, WA, a purified constituent of ASH, showed same pattern using MTT assay as a parameter. These results suggests that neuroprotective properties of ASH observed in the present study may provide some explanation for the ethnopharmacological uses of ASH in traditional medicine for cognitive and other HIV associated neurodegenerative disorders and further ASH could be a potential novel drug to reduce the brain amyloid burden and/or improve the HIV-1 associated neurocognitive impairments.
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