Transcriptome analysis reveals in vitro cultured Withania somnifera leaf and root tissues as a promising source for targeted withanolide biosynthesis
Senthil K, Jayakodi M, Thirugnanasambantham P, Lee SC, Duraisamy P, Purushotham PM, Rajasekaran K, Nancy Charles S, Mariam Roy I, Nagappan AK, Kim GS, Lee YS, Natesan S, Min TS, Yang TJ
BMC genomics · 20 citas
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- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- University Grants Commission
- Gobierno
- Rural Development Administration
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2015-01-21 · BMC Genomics · vol. 16 · número 1 · pág. 14
- Editorial
- BioMed Central
- Citado
- 61 citas · más que el 100% de estudios similares · 24.5× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 80 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Plant tissue culture and regeneration · Andrographolide Research and Applications
- Palabras clave
- Withanolide, Withania somnifera, Withaferin A, Biology, Transcriptome, UniGene, KEGG, Biochemistry, Gene expression, Gene
- MeSH
- cell line, tumor, humans, withania, plant leaves, plant roots, antioxidants, sequence analysis, rna, cell proliferation, cell survival, microsatellite repeats, expressed sequence tags, withanolides, transcriptome, cell cycle checkpoints
15 autores
- Kalaiselvi Senthil🇮🇳Avinashilingam University
- Murukarthick Jayakodi🇰🇷Seoul National University
- Pankajavalli Thirugnanasambantham🇮🇳Avinashilingam University
- Sang Choon Lee🇰🇷Seoul National University
- Pradeepa Duraisamy🇮🇳Avinashilingam University
- Preethi M. Purushotham🇮🇳Avinashilingam University
- Kalaiselvi Rajasekaran🇮🇳Avinashilingam University
- Shobana Nancy Charles🇮🇳Avinashilingam University
- Irene Mariam Roy🇮🇳Avinashilingam University
- Arul Kumar Nagappan🇰🇷Gyeongsang National University
- Gon Sup Kim🇰🇷Gyeongsang National University
- Yun Sun Lee🇰🇷Seoul National University
- Natesan Senthil🇮🇳Tamil Nadu Agricultural University
- Taesun Min🇰🇷National Research Foundation of Korea
- Tae‐Jin Yang🇰🇷Seoul National University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
The production of metabolites via in vitro culture is promoted by the availability of fully defined metabolic pathways. Withanolides, the major bioactive phytochemicals of Withania somnifera, have been well studied for their pharmacological activities. However, only a few attempts have been made to identify key candidate genes involved in withanolide biosynthesis. Understanding the steps involved in withanolide biosynthesis is essential for metabolic engineering of this plant to increase withanolide production.
Results
Transcriptome sequencing was performed on in vitro adventitious root and leaf tissues using the Illumina platform. We obtained a total of 177,156 assembled transcripts with an average unigene length of 1,033 bp. About 13% of the transcripts were unique to in vitro adventitious roots but no unique transcripts were observed in in vitro-grown leaves. A putative withanolide biosynthetic pathway was deduced by mapping the assembled transcripts to the KEGG database, and the expression of candidate withanolide biosynthesis genes -were validated by qRT PCR. The accumulation pattern of withaferin A and withanolide A varied according to the type of tissue and the culture period. Further, we demonstrated that in vitro leaf extracts exhibit anticancer activity against human gastric adenocarcinoma cell lines at sub G1 phase.
Conclusions
We report here a validated large-scale transcriptome data set and the potential biological activity of in vitro cultures of W. somnifera. This study provides important information to enhance tissue-specific expression and accumulation of secondary metabolites, paving the way for industrialization of in vitro cultures of W. somnifera.
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