Blueberry Component Pterostilbene Protects Corneal Epithelial Cells from Inflammation via Anti-oxidative Pathway
Li J, Ruzhi Deng, Hua X, Zhang L, Lu F, Coursey TG, Pflugfelder SC, Li DQ
Scientific reports · 86 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Organización sin fines de lucro
- Research to Prevent Blindness
- Gobierno
- National Institutes of Health
- Gobierno
- National Eye Institute
- Gobierno
- NEI NIH HHS
- Subvenciones
- National Eye Institute (R01 EY011915); National Eye Institute (P30 EY002520); National Eye Institute (R01EY023598); Research to Prevent Blindness (EY002520); National Eye Institute (EY002520); National Eye Institute (EY011915); National Eye Institute (EY023598); National Institutes of Health (R01 EY023598); National Institutes of Health (EY023598); National Institutes of Health (EY011915); National Institutes of Health (EY002520)
Según 4 financiadores declarados.
Publicación
- Publicado
- 2016-01-14 · Sci Rep · vol. 6 · número 1 · pág. 19408
- Editorial
- Nature Portfolio
- Citado
- 117 citas · más que el 99% de estudios similares · 11.1× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 54 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Corneal Surgery and Treatments · Ocular Surface and Contact Lens · Glaucoma and retinal disorders
- Palabras clave
- Pterostilbene, Oxidative stress, Reactive oxygen species, Inflammation, Superoxide dismutase, Chemistry, Proinflammatory cytokine, Antioxidant, Malondialdehyde, Corneal epithelium, Biochemistry, Pharmacology, Biology, Immunology, Cornea, Resveratrol
- MeSH
- epithelium, corneal, epithelial cells, humans, keratitis, reactive oxygen species, stilbenes, inflammation mediators, cytokines, antioxidants, signal transduction, gene expression, gene expression regulation, enzymologic, oxidation-reduction, oxidative stress, osmolar concentration, adult, aged, middle aged, young adult, blueberry plants
8 autores
- Jin Li🇺🇸🇨🇳Baylor College of Medicine; Wenzhou Medical University; Affiliated Eye Hospital of Wenzhou Medical College; Ocular Surface Center
- Ruzhi Deng🇺🇸🇨🇳Baylor College of Medicine; Wenzhou Medical University; Affiliated Eye Hospital of Wenzhou Medical College; Ocular Surface Center
- Xia Hua🇺🇸🇨🇳Baylor College of Medicine; Ocular Surface Center; Tianjin Medical University Eye Hospital; Tianjin Medical University
- Lili Zhang🇺🇸Baylor College of Medicine; Ocular Surface Center
- Fan Lü🇨🇳Wenzhou Medical University; Affiliated Eye Hospital of Wenzhou Medical College
- Terry G. Coursey🇺🇸Baylor College of Medicine; Ocular Surface Center
- Stephen Carl Pflugfelder🇺🇸Baylor College of Medicine; Ocular Surface Center
- De‐Quan Li🇺🇸Baylor College of Medicine; Ocular Surface Center
Resumen
El texto original del estudio, en el idioma en que se publicó.
Blueberries have been recognized to possess protective properties from inflammation and various diseases, but not for eye and ocular disorders. This study explores potential benefits of pterostilbene (PS), a natural component of blueberries, in preventing ocular surface inflammation using an in vitro culture model of human corneal epithelial cells (HCECs) exposed to hyperosmotic medium at 450 mOsM. Gene expression was detected by RT-qPCR, and protein production or activity was determined by ELISA, zymography, Western blotting and immunofluorescent staining. Reactive oxygen species (ROS) production was measured using DCFDA kit. The addition of PS significantly reduced the expression of pro-inflammatory mediators, TNF-α, IL-1 β, IL-6, MMP-2 and MMP-9 in HCECs exposed to hyperosmotic medium. Pre-treatment with PS (5 to 20 μM) suppressed ROS overproduction in a dose-dependent manner. Additionally, PS significantly decreased the levels of oxidative damage biomarkers, malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), aconitase-2 and 8-hydroxydeoxyguanosine (8-OHdG). Importantly, PS was found to rebalance homeostasis between oxygenases and anti-oxidative enzymes by decreasing cyclooxygenase 2 (COX2) expression and restoring the activity of antioxidant enzymes, superoxide dismutase 1 (SOD1) and peroxiredoxin-4 (PRDX4) during hyperosmotic stress. Our findings demonstrate that PS protects human cornea from hyperosmolarity-induced inflammation and oxidative stress, suggesting protective effects of PS on dry eye.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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