Effect of Broccoli Sprouts and Live Attenuated Influenza Virus on Peripheral Blood Natural Killer Cells: A Randomized, Double-Blind Study
Müller L, Meyer M, Bauer RN, Zhou H, Zhang H, Jones S, Robinette C, Noah TL, Jaspers I
PloS one · 38 citas
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- Financiación independiente
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- National Science Foundation
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- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
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- National Institutes of Health
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- National Heart, Lung, and Blood Institute
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- National Institute of General Medical Sciences
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- National Institute of Diabetes and Digestive and Kidney Diseases
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- National Institute of Environmental Health Sciences
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- NIGMS NIH HHS
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- Subvenciones
- National Institute of Environmental Health Sciences (P30-ES010126); National Institute of Environmental Health Sciences (T32 ES007126); National Institute of General Medical Sciences (K12GM000678); National Institute of Environmental Health Sciences (R01ES013611); National Institutes of Health (NIH-R01); National Institutes of Health (R01 HL095163); National Institute of Diabetes and Digestive and Kidney Diseases (P30DK056350); National Heart, Lung, and Blood Institute (R01HL095163)
Según 11 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2016-01-28 · PLoS One · vol. 11 · número 1 · pág. e0147742
- Editorial
- Public Library of Science
- Citado
- 49 citas · más que el 85% de estudios similares · 2.0× el promedio de su campo
- Referencias
- 39 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Immune Cell Function and Interaction · Genomics, phytochemicals, and oxidative stress · Exercise and Physiological Responses
- Palabras clave
- sulforaphane, natural killer cells, NKT cells, granzyme B, NK cell cytotoxicity, antiviral defense, dietary supplementation, peripheral immune cells, CD56, CD16
- MeSH
- killer cells, natural, humans, influenza a virus, brassica, medicago sativa, plant extracts, vaccines, attenuated, influenza vaccines, immunologic factors, lymphocyte count, double-blind method, adult, female, male, influenza, human, young adult
9 autores
- Loretta L. Müller🇺🇸🇨🇭University of North Carolina at Chapel Hill; University Children’s Hospital Basel
- Megan Meyer🇺🇸University of North Carolina at Chapel Hill
- Rebecca N. Bauer🇺🇸University of North Carolina at Chapel Hill; Stanford Medicine; Stanford University
- Haibo Zhou🇺🇸University of North Carolina at Chapel Hill
- Hongtao Zhang🇺🇸University of North Carolina at Chapel Hill
- Shannon Jones🇺🇸University of North Carolina at Chapel Hill
- Carole Robinette🇺🇸University of North Carolina at Chapel Hill
- Terry L. Noah🇺🇸University of North Carolina at Chapel Hill
- Ilona Jaspers🇺🇸 · autor de correspondenciaUniversity of North Carolina at Chapel Hill
Resumen
El texto original del estudio, en el idioma en que se publicó.
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Enhancing antiviral host defense responses through nutritional supplementation would be an attractive strategy in the fight against influenza. Using inoculation with live attenuated influenza virus (LAIV) as an infection model, we have recently shown that ingestion of sulforaphane-containing broccoli sprout homogenates (BSH) reduces markers of viral load in the nose. To investigate the systemic effects of short-term BSH supplementation in the context of LAIV-inoculation, we examined peripheral blood immune cell populations in non-smoking subjects from this study, with a particular focus on NK cells. We carried out a randomized, double-blinded, placebo-controlled study measuring the effects of BSH (N = 13) or placebo (alfalfa sprout homogenate, ASH; N = 16) on peripheral blood mononuclear cell responses to a standard nasal vaccine dose of LAIV in healthy volunteers. Blood was drawn prior to (day-1) and post (day2, day21) LAIV inoculation and analyzed for neutrophils, monocytes, macrophages, T cells, NKT cells, and NK cells. In addition, NK cells were enriched, stimulated, and assessed for surface markers, intracellular markers, and cytotoxic potential by flow cytometry. Overall, LAIV significantly reduced NKT (day2 and day21) and T cell (day2) populations. LAIV decreased NK cell CD56 and CD158b expression, while significantly increasing CD16 expression and cytotoxic potential (on day2). BSH supplementation further increased LAIV-induced granzyme B production (day2) in NK cells compared to ASH and in the BSH group granzyme B levels appeared to be negatively associated with influenza RNA levels in nasal lavage fluid cells. We conclude that nasal influenza infection may induce complex changes in peripheral blood NK cell activation, and that BSH increases virus-induced peripheral blood NK cell granzyme B production, an effect that may be important for enhanced antiviral defense responses.
Trial registration
ClinicalTrials.gov NCT01269723.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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