D, L-Sulforaphane Loaded Fe3O4@ Gold Core Shell Nanoparticles: A Potential Sulforaphane Delivery System
Kheiri Manjili H, Ma'mani L, Tavaddod S, Mashhadikhan M, Shafiee A, Naderi-Manesh H
PloS one · 34 citas
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- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
Según la declaración de financiación del texto completo.
Publicación
- Publicado
- 2016-03-16 · PLoS One · vol. 11 · número 3 · pág. e0151344
- Editorial
- Public Library of Science
- Citado
- 62 citas · más que el 92% de estudios similares · 3.2× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 39 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Synthesis and Characterization of Heterocyclic Compounds · Adenosine and Purinergic Signaling
- Palabras clave
- Sulforaphane, Polyethylene glycol, Nanoparticle, Nuclear chemistry, HeLa, Chemistry, Colloidal gold, Apoptosis, Cytotoxicity, Materials science, Nanotechnology, Biochemistry, In vitro
- MeSH
- humans, gold, ferric compounds, isothiocyanates, sulfoxides, microscopy, electron, drug delivery systems, spectroscopy, fourier transform infrared, spectrophotometry, ultraviolet, spectrometry, x-ray emission, apoptosis, metal nanoparticles, mcf-7 cells
6 autores
- Hamidreza Kheiri Manjili🇮🇷Tarbiat Modares University
- Leila Ma’mani🇮🇷Agricultural Research & Education Organization; Agricultural Biotechnology Research Institute of Iran
- Sharareh Tavaddod🇮🇷Tarbiat Modares University
- Maedeh Mashhadikhan🇮🇷Islamic Azad University, Science and Research Branch
- Abbas Shafiee🇮🇷 · autor de correspondenciaTehran University of Medical Sciences
- Hossein Naderi‐Manesh🇮🇷 · autor de correspondenciaTarbiat Modares University
Resumen
El texto original del estudio, en el idioma en que se publicó.
A novel design of gold-coated iron oxide nanoparticles was fabricated as a potential delivery system to improve the efficiency and stability of d, l-sulforaphane as an anticancer drug. To this purpose, the surface of gold-coated iron oxide nanoparticles was modified for sulforaphane delivery via furnishing its surface with thiolated polyethylene glycol-folic acid and thiolated polyethylene glycol-FITC. The synthesized nanoparticles were characterized by different techniques such as FTIR, energy dispersive X-ray spectroscopy, UV-visible spectroscopy, scanning and transmission electron microscopy. The average diameters of the synthesized nanoparticles before and after sulforaphane loading were obtained ∼ 33 nm and ∼ 38 nm, respectively, when ∼ 2.8 mmol/g of sulforaphane was loaded. The result of cell viability assay which was confirmed by apoptosis assay on the human breast cancer cells (MCF-7 line) as a model of in vitro-cancerous cells, proved that the bare nanoparticles showed little inherent cytotoxicity, whereas the sulforaphane-loaded nanoparticles were cytotoxic. The expression rate of the anti-apoptotic genes (bcl-2 and bcl-xL), and the pro-apoptotic genes (bax and bak) were quantified, and it was found that the expression rate of bcl-2 and bcl-xL genes significantly were decreased when MCF-7 cells were incubated by sulforaphane-loaded nanoparticles. The sulforaphane-loaded into the designed gold-coated iron oxide nanoparticles, acceptably induced apoptosis in MCF-7 cells.
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