Harnessing the Power of Cruciferous Vegetables: Developing a Biomarker for Brassica Vegetable Consumption Using Urinary 3,3'-Diindolylmethane
Fujioka N, Ransom BW, Carmella SG, Upadhyaya P, Lindgren BR, Roper-Batker A, Hatsukami DK, Fritz VA, Rohwer C, Hecht SS
Cancer prevention research (Philadelphia, Pa.) · 23 citas
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Alimentación controladaDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Sin resultado de salud
- Consumo medido con
- Comida provista por los investigadores
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institutes of Health
- Gobierno
- National Heart, Lung, and Blood Institute
- Gobierno
- National Cancer Institute
- Gobierno
- National Center for Advancing Translational Sciences
- Gobierno
- NCI NIH HHS
- Gobierno
- NHLBI NIH HHS
- Gobierno
- NIH
- Gobierno
- NHLBI
- Gobierno
- NCATS NIH HHS
- Subvenciones
- National Cancer Institute (P30 CA-077598); National Heart, Lung, and Blood Institute (T32 HL007062); National Heart, Lung, and Blood Institute (5T32HL007062-34); National Center for Advancing Translational Sciences (UL1 TR-000114); National Institutes of Health (P30 CA 77598)
Según 9 financiadores declarados.
Publicación
- Publicado
- 2016-08-18 · Cancer Prev Res (Phila) · vol. 9 · número 10 · págs. 788–793
- Editorial
- American Association for Cancer Research
- Citado
- 29 citas · más que el 88% de estudios similares · 2.3× el promedio de su campo
- Referencias
- 33 trabajos
- Acceso
- Acceso abierto (copia en repositorio)
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Bioactive Compounds and Antitumor Agents · Glutathione Transferases and Polymorphisms
- Palabras clave
- Brassica, Cruciferous vegetables, Urinary system, Urine, Metabolite, Chemistry, Food science, Biomarker, Medicine, Cancer, Biology, Internal medicine, Botany, Biochemistry
- MeSH
- humans, brassica, indoles, glucosinolates, chromatography, liquid, diet, adult, middle aged, tandem mass spectrometry, young adult, biomarkers
10 autores
- Naomi Fujioka🇺🇸 · autor de correspondenciaUniversity of Minnesota
- Benjamin W.S. Ransom🇺🇸University of Minnesota
- Steven G. Carmella🇺🇸University of Minnesota
- Pramod Upadhyaya🇺🇸University of Minnesota
- Bruce R. Lindgren🇺🇸University of Minnesota
- Astia Roper-Batker🇺🇸University of Vermont
- Dorothy K. Hatsukami🇺🇸University of Minnesota; University of Minnesota System
- Vincent A. Fritz🇺🇸University of Minnesota; University of Minnesota, Waseca
- Charles L. Rohwer🇺🇸University of Minnesota, Waseca
- Stephen S. Hecht🇺🇸University of Minnesota
Resumen
El texto original del estudio, en el idioma en que se publicó.
Glucobrassicin in Brassica vegetables gives rise to indole-3-carbinol (I3C), a compound with potent anticancer effects in preclinical models. We previously showed that the urinary metabolite 3,3'-diindolylmethane (DIM) could discriminate between volunteers fed high and low doses of Brassica vegetables. However, the quantitative relationship between glucobrassicin exposure and urinary DIM level is unclear. We conducted a clinical trial to examine the hypotheses that a range of glucobrassicin exposure from Brassica vegetables is reflected in urinary DIM and that this effect plateaus. Forty-five subjects consumed vegetables, a mixture of brussels sprouts and/or cabbage, at one of seven discrete dose levels of glucobrassicin ranging from 25 to 500 μmol, once daily for 2 consecutive days. All urine was collected for 24 hours after each vegetable-eating session. Urinary DIM was measured using our published liquid chromatography-electrospray ionization-tandem mass spectrometry-selected reaction monitoring (LC/ESI-MS/MS-SRM) method. Urinary DIM excretion increased predictably with increasing glucobrassicin dose and plateaued between 200 and 300 μmol of glucobrassicin. The association between glucobrassicin dose and urinary DIM was strong and positive (R2 = 0.68). The majority of DIM was excreted in the first 12 hours after vegetable consumption. We conclude that urinary DIM is a reliable biomarker of glucobrassicin exposure and I3C uptake and that feeding glucobrassicin beyond 200 μmol did not consistently lead to more urinary DIM, suggesting a plateau in potential chemopreventive benefit. Cancer Prev Res; 9(10); 788-93. ©2016 AACR.
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