Physiologic changes of urinary proteome by caffeine and excessive water intake
Peerapen P, Ausakunpipat N, Sutthimethakorn S, Aluksanasuwan S, Vinaiphat A, Thongboonkerd V
Clinical chemistry and laboratory medicine · 11 citas
Etiquetas de revisión
Financiación no declaradaSolo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Ensayo clínico controlado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación no declarada
Publicación
- Publicado
- 2016-12-17 · Clin Chem Lab Med · vol. 55 · número 7 · págs. 993–1002
- Editorial
- De Gruyter
- Citado
- 17 citas · más que el 85% de estudios similares · 1.7× el promedio de su campo
- Referencias
- 44 trabajos
- Acceso
- De pago
- Áreas de investigación
- Coffee research and impacts · Circadian rhythm and melatonin · Urinary Bladder and Prostate Research
- Palabras clave
- Urinary system, Proteome, Urine, Blot, Chemistry, Proteomics, Internal medicine, Endocrinology, Biochemistry, Medicine
- MeSH
- humans, water, caffeine, proteome, urinalysis, artifacts, drinking, dose-response relationship, drug, time factors, adult, female, male
6 autores
- Paleerath Peerapen🇹🇭Siriraj Hospital; Mahidol University
- Nardtaya Ausakunpipat🇹🇭Siriraj Hospital; Mahidol University
- Suchitra Sutthimethakorn🇹🇭Siriraj Hospital; Mahidol University
- Siripat Aluksanasuwan🇹🇭Siriraj Hospital; Mahidol University
- Arada Vinaiphat🇹🇭Siriraj Hospital; Mahidol University
- Visith Thongboonkerd🇹🇭 · autor de correspondenciaSiriraj Hospital; Mahidol University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Diurnal variations and physiologic changes of urinary proteome have been suggested in the urinary proteomics field. However, no clear evidence has been demonstrated. The present study thus aimed to define changes in urinary proteome by physiological stimuli, i.e. caffeine intake and excessive water drinking, both of which cause physiologic diuresis.
Methods
Urine samples were collected from 30 healthy individuals under three different conditions: (i) morning void as the control; (ii) after drinking a cup of coffee; and (iii) after drinking 1 L of water within 20 min. Thereafter, differentially excreted proteins were analyzed by 2-DE proteomics approach and validated by Western blotting and ELISA.
Results
Spot matching, quantitative intensity analysis, and ANOVA followed by Tukey's post-hoc multiple comparisons and the Bonferroni correction revealed significant differences in levels of five protein spots among three different conditions. These proteins were identified by quadrupole time-of-flight mass spectrometry (Q-TOF MS) and/or MS/MS analyses as kininogen 1 isoform 3, β-actin, prostaglandin D synthase (PGDS), fibrinogen α-chain and immunoglobulin light chain. Among these, the decreased level of immunoglobulin was successfully validated by Western blotting and ELISA.
Conclusions
These data indicated that caffeine intake and excessive water drinking could affect urinary excretion of some proteins and may affect urinary proteome analysis.
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