Low-Level Laser Effect on Proliferation, Migration, and Antiapoptosis of Mesenchymal Stem Cells
Yin K, Zhu R, Wang S, Zhao RC
Stem cells and development · 74 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Natural Science Foundation of China
Según 1 financiador declarado.
Publicación
- Publicado
- 2017-02-09 · Stem Cells Dev · vol. 26 · número 10 · págs. 762–775
- Editorial
- Mary Ann Liebert, Inc.
- Citado
- 92 citas · más que el 97% de estudios similares · 6.5× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 41 trabajos
- Acceso
- De pago
- Áreas de investigación
- Laser Applications in Dentistry and Medicine · Laser-Ablation Synthesis of Nanoparticles · Laser Material Processing Techniques
- Palabras clave
- Biology, Mesenchymal stem cell, Cell biology, Viability assay, Cell, Immunocytochemistry, Molecular biology, Biochemistry
- MeSH
- adipose tissue, cells, cultured, cytoskeleton, mitochondria, mesenchymal stem cells, humans, lasers, apoptosis, cell proliferation, cell movement, light, low-level light therapy
4 autores
- Kan Yin🇨🇳Chinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
- Rongjia Zhu🇨🇳Chinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
- Shihua Wang🇨🇳Chinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
- Robert Chunhua Zhao🇨🇳Chinese Academy of Medical Sciences & Peking Union Medical College; Peking Union Medical College Hospital
Resumen
El texto original del estudio, en el idioma en que se publicó.
Mesenchymal stem cells (MSCs) have been proved to be an important element in cell-based therapy. Photobiomodulation used extremely low-level lasers (LLLs) to affect the behavior of cells. The effect mechanism of LLLs on MSCs from human remained to be discovered. In this study, cell viability was assessed using MTS assays and cell cycle was evaluated by fluorescence-activated cell sorting (FACS). The influence of LLLs on mitochondrial biogenesis (fission or fusion) and function (ATP, reactive oxygen species [ROS], nitric oxide [NO]) was evaluated by transmission electron microscope, FACS, quantitative real time polymerase chain reaction (q-PCR), and immunocytochemistry. Cell migration and cytoskeleton alteration (actin and tubulin) were evaluated using transwell assay, immunocytochemistry, enzyme-linked immunosorbent assay, and western blotting. Cell apoptosis was evaluated using FACS, immunocytochemistry, and western blotting. We investigated that certain influence of LLLs on MSCs in vitro 6 or 24 h after 1 h of LLL irradiation. The mechanism of the effects included proliferation rate increase mediated by increased S phase proportion; mitochondrial biogenesis and function alteration mediated by fusion (Mfn1, Mfn2, and Opa-1) and fission (Fis1, Drp-1, and MTP18)-related proteins, NRF1, TFAM, PGC-1a, and upregulated intracellular ROS and NO concentration; migration acceleration through the ERK1/2 and FAK pathway and upregulation of growth factors such as HGF and PDGF; and resistance to apoptosis with increased Bcl-2 and decreased Bax, or through tunneling nanotube formation between LLL-treated MSCs and 5-fluorouracil-induced apoptotic MSCs. These observations suggested that LLLs enhanced stem cell survival and therapeutic function, which could appear to be an innovative pretreatment in the application of MSCs.
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