Cold exposure induces the acquisition of brown adipocyte gene expression profiles in cattle inguinal fat normalized with a new set of reference genes for qRT-PCR
Cao KX, Hao D, Wang J, Peng WW, Yan YJ, Cao HX, Sun F, Chen H
Research in veterinary science · 14 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Animales
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Posiblemente financiado por la industria
- Gobierno
- National Development and Reform Commission
- Grupo de la industria
- National Beef Cattle Industrial Technology System
- Gobierno
- National Beef Cattle and Yak Industrial Technology System
- Gobierno
- Science and Technology in Henan Province
- Gobierno
- Science and Technology Co-ordinator Innovative engineering projects of Shaanxi Province
- Subvenciones
- National Development and Reform Commission (2014–2573)
Según 5 financiadores declarados.
Publicación
- Publicado
- 2017-02-24 · Res Vet Sci · vol. 114 · págs. 1–5
- Editorial
- Elsevier BV
- Citado
- 16 citas · más que el 77% de estudios similares · 1.2× el promedio de su campo
- Referencias
- 36 trabajos
- Acceso
- De pago
- Áreas de investigación
- Adipose Tissue and Metabolism · Molecular Biology Techniques and Applications · Peroxisome Proliferator-Activated Receptors
- Palabras clave
- white adipose tissue browning, cold acclimation, reference genes, qRT-PCR normalization, HDAC3, mitochondrial biogenesis, thermoregulation, PGC-1α, UCP1, CITED1, Tbx1, NRF1, Nrf2, TFAM, gene expression profiling
- MeSH
- animals, cattle, humans, body weight, reverse transcriptase polymerase chain reaction, gene expression regulation, male, adipose tissue, brown, adipocytes, brown, cold temperature, transcriptome
8 autores
- K.X. Cao🇨🇳Northwest A&F University
- Dan Hao🇨🇳Northwest A&F University
- Junru Wang🇨🇳Northwest A&F University
- W.W. Peng🇨🇳Northwest A&F University
- Y.J. Yan🇨🇳Northwest A&F University
- Handi Cao
- F. Sun
- Hong Chen🇨🇳 · autor de correspondenciaNorthwest A&F University
Resumen
El texto original del estudio, en el idioma en que se publicó.
The last few years have seen great advances in our understanding of browning in white adipose tissue (WAT) where white adipocytes take on characteristics of brown adipocytes. At present, the economic significance of browning for animal husbandry is beginning to be realized with the emerging evidence that browning affects body weight not only in human and rodent but in farm animals. Quantitative RT-PCR provides a quick and sensitive way to preliminary determine browning of WAT. However, there have been no established condition specific reference genes for browning of cattle WAT. As the results showed, the most two stable reference genes for diet treatment were Wdr33 (M=0.38) and Hdac3 (M=0.43), while the most three internal controls for temperature treatment were Hdac3 (M=0.28), Wdr33 (M=0.32), and Hprt1 (M=0.39) among the ten candidates. The mRNA relative expression levels of selective marker genes were normalized by normalization factor (geometric mean of control genes quantities). Cold exposure rather than high energy diet induced transcript elevations of brite specific markers (Cited1, Tbx1), thermoregulatory markers (brown and beige versus white markers, i.e., Cidea, Cox7a1, Ucp1), mitochondrial biogenesis markers (Nrf1, Nrf2, Tfam), and transcription regulators (brown and beige versus white markers, i.e., Pgc1α) (P<0.05) in cattle inguinal fat (iWAT). Quantitative RT-PCR is a preliminary study for WAT browning. In conclusion, cattle inguinal fat acquired brown adipocyte gene expression features upon cold acclimation with prerequisite identification of stable reference genes.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.