Broccoli-Derived Nanoparticle Inhibits Mouse Colitis by Activating Dendritic Cell AMP-Activated Protein Kinase
Deng Z, Rong Y, Teng Y, Mu J, Zhuang X, Tseng M, Samykutty A, Zhang L, Yan J, Miller D, Suttles J, Zhang HG
Molecular therapy : the journal of the American Society of Gene Therapy · 391 citas
Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Animales
- Resultado principal
- Eventos clínicos como enfermedad o muerte
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- U.S. Department of Veterans Affairs
- Gobierno
- China Scholarship Council
- Gobierno
- National Institutes of Health
- Gobierno
- National Institute of General Medical Sciences
- Gobierno
- National Center for Advancing Translational Sciences
- Gobierno
- National Center for Complementary and Integrative Health
- Gobierno
- BLRD VA
- Gobierno
- NIGMS NIH HHS
- Gobierno
- NCCIH NIH HHS
- Gobierno
- Robley Rex VA Medical Center
- Gobierno
- NCATS NIH HHS
- Gobierno
- NIH
- Gobierno
- Research Career Scientist (RCS) Award
- Sin clasificar
- Institutional Development Award
- Subvenciones
- China Scholarship Council (201406275038); National Center for Complementary and Integrative Health (R01 AT008617); National Institute of General Medical Sciences (P20 GM113226); U.S. Department of Veterans Affairs (I01 BX000962); National Center for Advancing Translational Sciences (UH3TR000875); National Institute of General Medical Sciences (P20GM113226-6174); National Institutes of Health (UH3TR000875); National Institutes of Health (R01AT008617)
Según 14 financiadores declarados.
Publicación
- Publicado
- 2017-03-07 · Mol Ther · vol. 25 · número 7 · págs. 1641–1654
- Editorial
- Elsevier BV
- Citado
- 502 citas · más que el 96% de estudios similares · 5.0× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 49 trabajos
- Acceso
- Acceso abierto (revista híbrida) · CC-BY-NC-ND
- Áreas de investigación
- Immunotherapy and Immune Responses · Immune Cell Function and Interaction · Psoriasis: Treatment and Pathogenesis
- Palabras clave
- AMPK, Immune system, Colitis, Protein kinase A, Cell biology, Adoptive cell transfer, AMP-activated protein kinase, Cancer research, Biology, Chemistry, Immunology, Kinase, T cell
- MeSH
- dendritic cells, animals, mice, inbred c57bl, humans, mice, brassica, colitis, ulcerative, disease models, animal, sodium dodecyl sulfate, isothiocyanates, sulfoxides, lipids, anti-inflammatory agents, plant extracts, adoptive transfer, administration, oral, immune tolerance, gene expression, enzyme activation, nanoparticles, amp-activated protein kinases
12 autores
- Zhongbin Deng🇺🇸University of Louisville
- Yuan Rong🇺🇸University of Louisville
- Yun Teng🇺🇸University of Louisville
- Jingyao Mu🇺🇸University of Louisville
- Xiaoying Zhuang🇺🇸University of Louisville
- Michael T. Tseng🇺🇸University of Louisville
- Abhilash Kumble Samykutty🇺🇸University of Louisville
- Lifeng Zhang🇺🇸University of Louisville
- Jun Yan🇺🇸University of Louisville
- Donald Max Miller🇺🇸University of Louisville
- Jill Suttles🇺🇸University of Louisville
- Huang‐Ge Zhang🇺🇸 · autor de correspondenciaUniversity of Louisville; Louisville VA Medical Center; James Graham Brown Foundation
Resumen
El texto original del estudio, en el idioma en que se publicó.
The intestinal immune system is continuously exposed to massive amounts of nanoparticles derived from food. Whether nanoparticles from plants we eat daily have a role in maintaining intestinal immune homeostasis is poorly defined. Here, we present evidence supporting our hypothesis that edible nanoparticles regulate intestinal immune homeostasis by targeting dendritic cells (DCs). Using three mouse colitis models, our data show that orally given nanoparticles isolated from broccoli extracts protect mice against colitis. Broccoli-derived nanoparticle (BDN)-mediated activation of adenosine monophosphate-activated protein kinase (AMPK) in DCs plays a role in not only prevention of DC activation but also induction of tolerant DCs. Adoptively transferring DCs pre-pulsed with total BDN lipids, but not sulforaphane (SFN)-depleted BDN lipids, prevented DSS-induced colitis in C57BL/6 (B6) mice, supporting the role of BDN SFN in the induction of DC tolerance. Adoptively transferring AMPK+/+, but not AMPK-/-, DCs pre-pulsed with SFN prevented DSS-induced colitis in B6 mice, further supporting the DC AMPK role in SFN-mediated prevention of DSS-induced colitis. This finding could open new preventive or therapeutic avenues to address intestinal-related inflammatory diseases via activating AMPK.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY-NC-ND).
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