A Fermented Whole Grain Prevents Lipopolysaccharides-Induced Dysfunction in Human Endothelial Progenitor Cells
Giusti L, Gabriele M, Penno G, Garofolo M, Longo V, Del Prato S, Lucchesi D, Pucci L
Oxidative medicine and cellular longevity · 28 citas
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- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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- Resultado principal
- Solo mecanismos
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- Financiación
- Financiación independiente
- Gobierno
- Regione Toscana
- Subvenciones
- Regione Toscana (D55E11002680005)
Según 1 financiador declarado y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2017-01-01 · Oxid Med Cell Longev · vol. 2017 · número 1 · pág. 1026268
- Editorial
- Hindawi Publishing Corporation
- Citado
- 40 citas · más que el 81% de estudios similares · 1.6× el promedio de su campo
- Referencias
- 49 trabajos
- Acceso
- Acceso abierto (revista híbrida) · CC-BY
- Áreas de investigación
- Immune cells in cancer · Adipokines, Inflammation, and Metabolic Diseases · Inflammation biomarkers and pathways
- Palabras clave
- Proinflammatory cytokine, Progenitor cell, Cell biology, Unfolded protein response, Oxidative stress, Viability assay, Endothelial dysfunction, Inflammation, Chemistry, NF-κB, Signal transduction, Apoptosis, Stem cell, Endoplasmic reticulum, Biology, Immunology, Endocrinology, Biochemistry
- MeSH
- leukocytes, mononuclear, humans, reactive oxygen species, lipopolysaccharides, plant preparations, cell survival, fermentation, real-time polymerase chain reaction, endothelial progenitor cells, whole grains
8 autores
- Laura Giusti🇮🇹University of Pisa; Azienda Ospedaliera Universitaria Pisana
- Morena Gabriele🇮🇹National Research Council; Deleted Institution
- Giuseppe Penno🇮🇹University of Pisa; Azienda Ospedaliera Universitaria Pisana
- Monia Garofolo🇮🇹University of Pisa; Azienda Ospedaliera Universitaria Pisana
- Vincenzo G. Longo🇮🇹 · autor de correspondenciaNational Research Council; Deleted Institution
- Stefano Del Prato🇮🇹University of Pisa; Azienda Ospedaliera Universitaria Pisana
- Daniela Lucchesi🇮🇹University of Pisa; Azienda Ospedaliera Universitaria Pisana
- Laura Pucci🇮🇹National Research Council; Deleted Institution
Resumen
El texto original del estudio, en el idioma en que se publicó.
Endogenous and exogenous signals derived by the gut microbiota such as lipopolysaccharides (LPS) orchestrate inflammatory responses contributing to development of the endothelial dysfunction associated with atherosclerosis in obesity, metabolic syndrome, and diabetes. Endothelial progenitor cells (EPCs), bone marrow derived stem cells, promote recovery of damaged endothelium playing a pivotal role in cardiovascular repair. Since healthy nutrition improves EPCs functions, we evaluated the effect of a fermented grain, Lisosan G (LG), on early EPCs exposed to LPS. The potential protective effect of LG against LPS-induced alterations was evaluated as cell viability, adhesiveness, ROS production, gene expression, and NF-kB signaling pathway activation. Our results showed that LPS treatment did not affect EPCs viability and adhesiveness but induced endothelial alterations via activation of NF-kB signaling. LG protects EPCs from inflammation as well as from LPS-induced oxidative and endoplasmic reticulum (ER) stress reducing ROS levels, downregulating proinflammatory and proapoptotic factors, and strengthening antioxidant defense. Moreover, LG pretreatment prevented NF-kB translocation from the cytoplasm into the nucleus caused by LPS exposure. In human EPCs, LPS increases ROS and upregulates proinflammatory tone, proapoptotic factors, and antioxidants. LG protects EPCs exposed to LPS reducing ROS, downregulating proinflammatory and proapoptotic factors, and strengthening antioxidant defenses possibly by inhibiting NF-κB nuclear translocation.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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