Withania somnifera Extract Protects Model Neurons from In Vitro Traumatic Injury
Saykally JN, Hatic H, Keeley KL, Jain SC, Ravindranath V, Citron BA
Cell transplantation · 16 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- U.S. Department of Veterans Affairs
- Gobierno
- RRD VA
- Subvenciones
- U.S. Department of Veterans Affairs (I01 RX001520)
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2017-06-30 · Cell Transplant · vol. 26 · número 7 · págs. 1193–1201
- Editorial
- SAGE Publishing
- Citado
- 20 citas · más que el 87% de estudios similares · 2.3× el promedio de su campo
- Referencias
- 43 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY-NC
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Medicinal Plants and Neuroprotection
- Palabras clave
- Withania somnifera, Neuroprotection, Neurite, Traumatic brain injury, Lactate dehydrogenase, Pharmacology, Apoptosis, Annexin, Programmed cell death, Medicine, CREB, Neuron, Biology, In vitro, Transcription factor, Neuroscience, Pathology, Biochemistry
- MeSH
- neurons, neurites, cell line, tumor, humans, withania, plant roots, wounds and injuries, neuroprotective agents, plant extracts, antioxidants, protein array analysis, signal transduction, apoptosis, cell survival, models, biological, nf-e2-related factor 2
6 autores
- Jessica N. Saykally🇺🇸Bay Pines VA Healthcare System
- Haris Hatic🇺🇸Bay Pines VA Healthcare System
- Kristen L. Keeley🇺🇸Bay Pines VA Healthcare System
- Subhash Chand Jain🇮🇳University of Delhi
- Vijayalakshmi Ravindranath🇮🇳Indian Institute of Science Bangalore
- Bruce A. Citron🇺🇸 · autor de correspondenciaBay Pines VA Healthcare System
Resumen
El texto original del estudio, en el idioma en que se publicó.
Withania somnifera has been used in traditional medicine for a variety of neural disorders. Recently, chronic neurodegenerative conditions have been shown to benefit from treatment with this extract. To evaluate the action of this extract on traumatically injured neurons, the efficacy of W. somnifera root extract as a neuroprotective agent was examined in cultured model neurons exposed to an in vitro injury system designed to mimic mild traumatic brain injury (TBI). Neuronal health was evaluated by staining with annexin V (an early, apoptotic feature) and monitoring released lactate dehydrogenase activity (a terminal cell loss parameter). Potential mechanisms underlying the observed neuroprotection were examined. Additionally, morphological changes were monitored following injury and treatment. Although no differences were found in the expression of the antioxidant transcription factor nuclear factor erythroid 2-like 2 (Nrf2) or other Nrf2-related downstream components, significant changes were seen in apoptotic signaling. Treatment with the extract resulted in an increased length of neurites projecting from the neuronal cell body after injury. W. somnifera extract treatment also resulted in reduced cell death in the model neuron TBI system. The cell death factor Bax was involved (its expression was reduced 2-fold by the treatment) and injury-induced reduction in neurite lengths and numbers was reversed by the treatment. This all indicates that W. somnifera root extract was neuroprotective and could have therapeutic potential to target factors involved in secondary injury and long-term sequelae of mild TBI.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY-NC).
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