Anti-inflammatory effects of low-level laser therapy on human periodontal ligament cells: in vitro study
Lee JH, Chiang MH, Chen PH, Ho ML, Lee HE, Wang YH
Lasers in medical science · 44 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- Ministry of Science and Technology, Taiwan
- Universidad u hospital
- Kaohsiung Medical University
- Subvenciones
- Ministry of Science and Technology, Taiwan (MOST-104-2314-B-037-059); Ministry of Science and Technology, Taiwan (MOST 104?); Kaohsiung Medical University (KMU-TP103B08); Kaohsiung Medical University (KMU-TP104B11)
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2017-11-07 · Lasers Med Sci · vol. 33 · número 3 · págs. 469–477
- Editorial
- Springer Science+Business Media
- Citado
- 67 citas · más que el 94% de estudios similares · 3.7× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 36 trabajos
- Acceso
- Acceso abierto (revista híbrida) · CC-BY
- Áreas de investigación
- Laser Applications in Dentistry and Medicine · Oral microbiology and periodontitis research · Connexins and lens biology
- Palabras clave
- Periodontal fiber, Low level laser therapy, Tumor necrosis factor alpha, Inflammation, Lipopolysaccharide, Intracellular, Cytokine, Chemistry, Panax notoginseng, Interleukin, Pharmacology, Medicine, Immunology, Pathology, Dentistry, Biochemistry, Laser therapy
- MeSH
- periodontal ligament, humans, inflammation, adenine, lipopolysaccharides, nf-kappa b, cyclic amp, anti-inflammatory agents, inflammation mediators, cytokines, cell death, cell survival, transcription, genetic, gene expression regulation, low-level light therapy
6 autores
- Ji-Hua Lee🇹🇼Kaohsiung Medical University
- Min‐Hsuan Chiang🇹🇼Kaohsiung Medical University
- Ping‐Ho Chen🇹🇼National Sun Yat-sen University; Kaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
- Mei‐Ling Ho🇹🇼National Sun Yat-sen University; Kaohsiung Medical University
- Huey-Er Lee🇹🇼Kaohsiung Medical University; Kaohsiung Medical University Chung-Ho Memorial Hospital
- Yan‐Hsiung Wang🇹🇼 · autor de correspondenciaKaohsiung Medical University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Periodontal disease is a chronic inflammatory disease that is commonly treated with surgical and nonsurgical techniques. However, both approaches have limitations. Low-level laser therapy (LLLT) has been widely applied in reducing inflammatory reactions, and research indicates that LLLT induces an anti-inflammatory effect that may enhance periodontal disease therapy. The purpose of this study was to investigate the anti-inflammatory effect of LLLT on human periodontal ligament cells (hPDLCs) in an inflammatory environment and aimed to determine the possible mechanism of action. Cells were cultured and treated with or without lipopolysaccharide (LPS) from Porphryromonas gingivalis or Escherichia coli, followed by irradiation with a gallium-aluminum-arsenide (GaAlAs) laser (660 nm) at an energy density of 8 J/cm2. Quantitative real-time polymerase chain reactions were used to assess the expression of pro-inflammatory genes, including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, and IL-8. The dual-luciferase reporter assay was used to examine nuclear factor-κB (NF-κB) transcriptional activity. An enzyme-linked immunosorbent assay was used to monitor the concentration of intracellular cyclic adenosine monophosphate (cAMP). Both LPS treatments significantly induced the mRNA expression of pro-inflammatory cytokines. However, LLLT inhibited the LPS-induced pro-inflammatory cytokine expression and elevated intracellular levels of cAMP. The LLLT inhibitory effect may function by downregulating NF-κB transcriptional activity and by increasing the intracellular levels of cAMP. LLLT might inhibit LPS-induced inflammation in hPDLCs through cAMP/NF-κB regulation. These results should be further studied to improve periodontal therapy.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.