Cytotoxic Effects and Anti-Angiogenesis Potential of Pistachio (Pistacia vera L.) Hulls against MCF-7 Human Breast Cancer Cells
Seifaddinipour M, Farghadani R, Namvar F, Mohamad J, Abdul Kadir H
Molecules (Basel, Switzerland) · 24 citas
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- Financiación independiente
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Publicación
- Publicado
- 2018-01-05 · Molecules · vol. 23 · número 1 · pág. 110
- Editorial
- Multidisciplinary Digital Publishing Institute
- Citado
- 55 citas · más que el 92% de estudios similares · 3.3× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 26 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Nuts composition and effects · Tannin, Tannase and Anticancer Activities · Research in Cotton Cultivation
- Palabras clave
- Pistacia, MCF-7, Human breast, Angiogenesis, Cytotoxic T cell, Cancer, Breast cancer, Traditional medicine, Biology, Cancer research, Botany, Medicine, Internal medicine, In vitro, Biochemistry
- MeSH
- chorioallantoic membrane, hct116 cells, ht29 cells, chick embryo, animals, humans, pistacia, breast neoplasms, angiogenesis inhibitors, plant extracts, antineoplastic agents, phytogenic, drug screening assays, antitumor, inhibitory concentration 50, apoptosis, cell shape, cell survival, neovascularization, physiologic, female, apoptosis regulatory proteins, mcf-7 cells
5 autores
- Maryam Seifaddinipour🇲🇾University of Malaya
- Reyhaneh Farghadani🇲🇾University of Malaya
- Farideh Namvar🇮🇷 · autor de correspondenciaIslamic Azad University, Mashhad
- Jamaludin Mohamad🇲🇾University of Malaya
- Habsah Abdul Kadir🇲🇾 · autor de correspondenciaUniversity of Malaya
Resumen
El texto original del estudio, en el idioma en que se publicó.
Pistachio (Pistacia vera L.) hulls (PVLH) represents a significant by-product of industrial pistachio processing that contains high amounta of phenolic and flavonoid compounds known to act as antioxidants. The current study was designed to evaluate the anti-tumor and anti-angiogenic potentials of PVLH extracts. The cytotoxic effects of hexane, ethyl acetate, methanol, and water PVLH extracts toward human colon cancer (HT-29 and HCT-116), breast adenocarcinoma (MCF-7), lung adenocarcinoma (H23), liver hepatocellular carcinoma (HepG2), cervical cancer (Ca Ski), and normal fibroblast (BJ-5ta) cells were assessed using a MTT cell viability assay. Apoptosis induction was evaluated through the different nuclear staining assays and confirmed by flow cytometry analysis. Anti-angiogenic activities were also determined using chorioallantoic membrane (CAM) assay. PVLH ethyl acetate extracts (PVLH-EAE) demonstrated a suppressive effect with an IC50 value of 21.20 ± 1.35, 23.00 ± 1.2 and 25.15 ± 1.85 µg/mL against MCF-7, HT-29 and HCT-116, respectively, after 72 h of treatment. Morphological assessment and flow cytometry analysis showed the potential of PVLH-EAE to induce apoptosis. PVLH-EAE at the highest concentration demonstrated significant inhibition of angiogenesis as comparing with control group. Also the expression of Bax increased and the expression of Bcl-2 decreased in treated MCF-7 cells. Thus, the apoptosis induction and angiogenesis potential of PVLH-EAE make it to be the most suitable for further cancer research study to deal with selective antitumor active substances to human cancers especially breast cancer.
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