The standardized Withania somnifera Dunal root extract alters basal and morphine-induced opioid receptor gene expression changes in neuroblastoma cells
Caputi FF, Acquas E, Kasture S, Ruiu S, Candeletti S, Romualdi P
BMC complementary and alternative medicine · 10 citas
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Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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- Resultado principal
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Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- Università di Bologna
- Gobierno
- Regione Autonoma della Sardegna
- Subvenciones
- Università di Bologna (RFO 2014); Regione Autonoma della Sardegna (CUP B71J11001480002); Regione Autonoma della Sardegna (RAS CRP 26805-CUP B71J11001480002.)
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2018-01-10 · BMC Complement Altern Med · vol. 18 · número 1 · pág. 9
- Editorial
- BioMed Central
- Citado
- 19 citas · más que el 90% de estudios similares · 3.4× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 63 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Neuropeptides and Animal Physiology · Pain Mechanisms and Treatments
- Palabras clave
- Pharmacology, Withania somnifera, Opioid, Morphine, (+)-Naloxone, NOP, Receptor, Opioid receptor, Nociceptin receptor, μ-opioid receptor, Medicine, Gene expression, SH-SY5Y, Chemistry, Opioid peptide, Biology, Internal medicine, Neuroblastoma, Cell culture, Gene, Biochemistry
- MeSH
- cell line, tumor, humans, withania, plant roots, neuroblastoma, receptors, opioid, plant extracts, cell survival, gene expression regulation, neoplastic, real-time polymerase chain reaction
6 autores
- Francesca Felicia Caputi🇮🇹 · autor de correspondenciaUniversity of Bologna
- Elio Acquas🇮🇹University of Cagliari
- Sanjay Bhaskar Kasture
- Stefania Ruiu🇮🇹Istituto di Farmacologia Traslazionale; National Research Council
- Sanzio Candeletti🇮🇹University of Bologna
- Patrizia Romualdi🇮🇹University of Bologna
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Behavioral studies demonstrated that the administration of Withania somnifera Dunal roots extract (WSE), prolongs morphine-elicited analgesia and reduces the development of tolerance to the morphine's analgesic effect; however, little is known about the underpinning molecular mechanism(s). In order to shed light on this issue in the present paper we explored whether WSE promotes alterations of μ (MOP) and nociceptin (NOP) opioid receptors gene expression in neuroblastoma SH-SY5Y cells.
Methods
A range of WSE concentrations was preliminarily tested to evaluate their effects on cell viability. Subsequently, the effects of 5 h exposure to WSE (0.25, 0.50 and 1.00 mg/ml), applied alone and in combination with morphine or naloxone, on MOP and NOP mRNA levels were investigated.
Results
Data analysis revealed that morphine decreased MOP and NOP receptor gene expression, whereas naloxone elicited their up-regulation. In addition, pre-treatment with naloxone prevented the morphine-elicited gene expression alterations. Interestingly, WSE was able to: a) alter MOP but not NOP gene expression; b) counteract, at its highest concentration, morphine-induced MOP down-regulation, and c) hamper naloxone-induced MOP and NOP up-regulation.
Conclusion
Present in-vitro data disclose novel evidence about the ability of WSE to influence MOP and NOP opioid receptors gene expression in SH-SY5Y cells. Moreover, our findings suggest that the in-vivo modulation of morphine-mediated analgesia by WSE could be related to the hindering of morphine-elicited opioid receptors down-regulation here observed following WSE pre-treatment at its highest concentration.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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