Financiado en parte por U.S. Highbush Blueberry Council, US Highbush Blueberry Council
Dual action of highbush blueberry proanthocyanidins on Aggregatibacter actinomycetemcomitans and the host inflammatory response
Ben Lagha A, LeBel G, Grenier D
BMC complementary and alternative medicine · 30 citas
Etiquetas de revisión
Financiado por la industriaSolo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiado por la industria
- Grupo de la industria
- U.S. Highbush Blueberry Council
- Universidad u hospital
- Universitetet i Oslo
- Grupo de la industria
- US Highbush Blueberry Council
Según 3 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2018-01-10 · BMC Complement Altern Med · vol. 18 · número 1 · pág. 10
- Editorial
- BioMed Central
- Citado
- 44 citas · más que el 89% de estudios similares · 2.4× el promedio de su campo
- Referencias
- 97 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Bacterial biofilms and quorum sensing · Antimicrobial Resistance in Staphylococcus · Phytochemicals and Antioxidant Activities
- Palabras clave
- Aggregatibacter actinomycetemcomitans, Viability assay, Microbiology, Biofilm, Grape seed extract, Vaccinium, Porphyromonas gingivalis, Macrophage, Medicine, Biology, Cell, In vitro, Biochemistry, Periodontitis, Bacteria, Pathology, Internal medicine
- MeSH
- u937 cells, tight junctions, macrophages, keratinocytes, humans, periodontitis, proanthocyanidins, nf-kappa b, plant extracts, cytokines, host-pathogen interactions, aggregatibacter actinomycetemcomitans, blueberry plants
3 autores
- Amel Ben Lagha🇨🇦Université Laval
- Geneviève LeBel🇨🇦Université Laval
- Daniel Grenier🇨🇦 · autor de correspondenciaNatural Sciences and Engineering Research Council of Canada; Université Laval
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
The highbush blueberry (Vaccinium corymbosum) has a beneficial effect on several aspects of human health. The present study investigated the effects of highbush blueberry proanthocyanidins (PACs) on the virulence properties of Aggregatibacter actinomycetemcomitans and macrophage-associated inflammatory responses.
Methods
PACs were isolated from frozen highbush blueberries using solid-phase chromatography. A microplate dilution assay was performed to determine the effect of highbush blueberry PACs on A. actinomycetemcomitans growth as well as biofilm formation stained with crystal violet. Tight junction integrity of oral keratinocytes was assessed by measuring the transepithelial electrical resistance (TER), while macrophage viability was determined with a colorimetric MTT assay. Pro-inflammatory cytokine and MMP secretion by A. actinomycetemcomitans-stimulated macrophages was quantified by ELISA. The U937-3xκB-LUC monocyte cell line transfected with a luciferase reporter gene was used to monitor NF-κB activation.
Results
Highbush blueberry PACs reduced the growth of A. actinomycetemcomitans and prevented biofilm formation at sub-inhibitory concentrations. The treatment of pre-formed biofilms with the PACs resulted in a loss of bacterial viability. The antibacterial activity of the PACs appeared to involve damage to the bacterial cell membrane. The PACs protected the oral keratinocytes barrier integrity from damage caused by A. actinomycetemcomitans. The PACs also protected macrophages from the deleterious effect of leukotoxin Ltx-A and dose-dependently inhibited the secretion of pro-inflammatory cytokines (IL-1β, IL-6, CXCL8, TNF-α), matrix metalloproteinases (MMP-3, MMP-9), and sTREM-1 by A. actinomycetemcomitans-treated macrophages. The PACs also inhibited the activation of the NF-κB signaling pathway.
Conclusion
The antibacterial and anti-inflammatory properties of highbush blueberry PACs as well as their ability to protect the oral keratinocyte barrier and neutralize leukotoxin activity suggest that they may be promising candidates as novel therapeutic agents.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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