Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's)
Naidoo DB, Chuturgoon AA, Phulukdaree A, Guruprasad KP, Satyamoorthy K, Sewram V
BMC complementary and alternative medicine · 16 citas
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- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
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- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Research Foundation
- Gobierno
- South African Medical Research Council
- Universidad u hospital
- Manipal University
- Gobierno
- Medical Research Council
- Gobierno
- Department of Science and Technology, Government of India
Según 5 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2018-04-10 · BMC Complement Altern Med · vol. 18 · número 1 · pág. 126
- Editorial
- BioMed Central
- Citado
- 31 citas · más que el 94% de estudios similares · 4.9× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 81 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Phytochemicals and Medicinal Plants · Inflammasome and immune disorders · MicroRNA in disease regulation
- Palabras clave
- Withania somnifera, Peripheral blood mononuclear cell, Viability assay, Tumor necrosis factor alpha, Medicine, Glutathione, Pharmacology, Apoptosis, Immunology, Chemistry, Biochemistry, In vitro, Enzyme, Pathology
- MeSH
- leukocytes, mononuclear, cells, cultured, humans, withania, neoplasms, cachexia, caspases, plant extracts, cytokines, apoptosis, cell survival, thp-1 cells
6 autores
- Dhaneshree Bestinee Naidoo🇿🇦University of KwaZulu-Natal
- Anil Amichund Chuturgoon🇿🇦University of KwaZulu-Natal
- Alisa Phulukdaree🇿🇦University of KwaZulu-Natal
- Kanive Parashiva Guruprasad🇮🇳Manipal Academy of Higher Education
- Kapaettu Satyamoorthy🇮🇳Manipal Academy of Higher Education
- Vikash Sewram🇿🇦 · autor de correspondenciaWestern Cape Department of Health; Stellenbosch University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
Cancer and inflammation are associated with cachexia. Withania somnifera (W. somnifera) possesses antioxidant and anti-inflammatory potential. We investigated the potential of an aqueous extract of the root of W. somnifera (WRE) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's).
Methods
Cytotoxcity of WRE was determined at 24 and 72 h (h). Oxidant scavenging activity of WRE was evaluated (2, 2-diphenyl-1 picrylhydrazyl assay). Glutathione (GSH) levels, caspase (- 8, - 9, - 3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were thereafter assayed. Tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 levels were also assessed using enzyme-linked immunosorbant assay.
Results
At 24 h, WRE (0.2-0.4 mg/ml) decreased PBMC viability between 20 and 25%, whereas it increased THP-1 viability between 15 and 23% (p RE increased PBMC viability by 27-39% (0.05, 0.4 mg/ml WRE) whereas decreased THP-1 viability between 9 and 16% (0.05-0.4 mg/ml WRE) (p RE (0.05-0.4 mg/ml, p RE, whereas IL-1β levels were increased by 0.05-0.4 mg/ml WRE (p RE (0.05-0.4 mg/ml) decreased TNF-α, IL-1β and IL-6 levels (p RE (p RE (0.1-0.4 mg/ml) decreased GSH levels in both cell lines (p RE (0.2-0.4 mg/ml) increased PBMC caspase (-8, -3/7) activities whereas WRE (0.05, 0.1, 0.4 mg/ml) increased THP-1 caspase (-9, -3/7) activities (p RE (p RE, whereas decreased by 0.05 and 0.4 mg/ml WRE (72 h, p < 0.0001).
Conclusion
In PBMC's and THP-1 cells, WRE proved to effectively modulate antioxidant activity, inflammatory cytokines and cell death. In THP-1 cells, WRE decreased pro-inflammatory cytokine levels, which may alleviate cancer cachexia and excessive leukaemic cell growth.
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