A short-term increase in dietary cholesterol and fat intake affects high-density lipoprotein composition in healthy subjects
Morgantini C, Trifirò S, Tricò D, Meriwether D, Baldi S, Mengozzi A, Reddy ST, Natali A
Nutrition, metabolism, and cardiovascular diseases : NMCD · 12 citas
Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Marcadores de salud y funcionamiento
- Consumo medido con
- No se indica
Quién lo pagó
- Financiación
- Financiación independiente
- Organización sin fines de lucro
- Fondation Leducq
- Universidad u hospital
- Università di Pisa
- Gobierno
- National Institutes of Health
- Gobierno
- U.S. Public Health Service
- Gobierno
- NIH HHS
- Organización sin fines de lucro
- Leducq Foundation
- Universidad u hospital
- University of Pisa (Fondi di Ateneo)
- Gobierno
- U.S. Public Health Service Research Grants
- Subvenciones
- U.S. Public Health Service (HL30568); National Institutes of Health (HL30568)
Según 8 financiadores declarados.
Publicación
- Publicado
- 2018-03-19 · Nutr Metab Cardiovasc Dis · vol. 28 · número 6 · págs. 575–581
- Editorial
- Elsevier BV
- Citado
- 17 citas · más que el 82% de estudios similares · 1.6× el promedio de su campo
- Referencias
- 36 trabajos
- Acceso
- De pago
- Áreas de investigación
- Cholesterol and Lipid Metabolism · Paraoxonase enzyme and polymorphisms · Fatty Acid Research and Health
- Palabras clave
- high-density lipoprotein, dietary cholesterol, dietary fat, HDL dysfunction, lipid hydroperoxides, hydroxyeicosatetraenoic acids, hydroxyoctadecadienoic acids, serum amyloid A, haptoglobin, HDL proteome, isocaloric diet
- MeSH
- humans, lipid peroxides, cholesterol, dietary, hydroxyeicosatetraenoic acids, linoleic acids, lipoproteins, hdl, serum amyloid a protein, prospective studies, cross-over studies, postprandial period, time factors, adult, italy, female, male, young adult, diet, high-fat, healthy volunteers, biomarkers
8 autores
- Cecilia Morgantini🇮🇹🇸🇪University of Pisa; Karolinska Institutet; Integrated Cardio Metabolic Centre
- Silvia Trifirò🇮🇹University of Pisa
- Domenico Tricò🇮🇹University of Pisa; Scuola Superiore Sant'Anna
- David Meriwether🇺🇸University of California, Los Angeles
- Simona Baldi🇮🇹University of Pisa
- Alessandro Mengozzi🇮🇹University of Pisa
- Srinivasa T. Reddy🇺🇸University of California, Los Angeles
- Andrea Natali🇮🇹 · autor de correspondenciaUniversity of Pisa
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background and aims
High-cholesterol and high-fat diets alter biochemical composition and anti-oxidant properties of high-density lipoproteins (HDL) in animals. Whether this occurs in humans is unknown. Therefore, we examined the effect of a short-term elevation in dietary cholesterol and fat intake on HDL composition in healthy subjects.
Methods and results
In a randomized, crossover clinical trial, 14 healthy young volunteers followed a 14-day low-cholesterol/low-fat diet (LChF) and a 14-day isocaloric high-cholesterol/high-fat diet (HChF) in a random order. After each diet, we measured HDL concentrations of hydroxyeicosatetraenoic acids (HETE), hydroxyoctadecadienoic acids (HODE), and haptoglobin, as well as serum amyloid A (SAA) and paroxonase-1 activity (PON-1). HDL concentrations of 15-HETE (+254%, p = 0.002), 5-HETE (+116%, p = 0.004), 13-HODE (+102%, p = 0.049), and SAA levels (+75%, p = 0.007) were significantly higher after the HChF than after the LChF. Furthermore, haptoglobin was marginally increased (+32%, p = 0.091) while PON-1 activity was unaffected (-16%, p = 0.366) by the HChF.
Conclusion
In healthy subjects, a short-term elevation in dietary cholesterol and fat intake increases HDL lipid hydroperoxide content (15-HETE, 5-HETE, 13-HODE) and SAA levels, which are key features of dysfunctional HDL. This is the first study showing that a physiologic manipulation of dietary cholesterol and fat intake affects HDL lipidome and proteome in healthy subjects independently of weight changes.
Clinical trial registration
NCT02549144.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
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