An integrated framework using high-dimensional mass cytometry and fluorescent flow cytometry identifies discrete B cell subsets in patients with red meat allergy
Cox KM, Commins SP, Capaldo BJ, Workman LJ, Platts-Mills TAE, Amir ED, Lannigan JA, Schuyler AJ, Erickson LD
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 14 citas
Etiquetas de revisión
Solo mecanismosSin ajuste declarado por estilo de vidaDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio de casos y controles (indexado por PubMed)
- Estudiado en
- Personas
- Resultado principal
- Solo mecanismos
- Consumo medido con
- No se indica
Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- University of Virginia
- Gobierno
- National Institutes of Health
- Gobierno
- National Institute of Allergy and Infectious Diseases
- Gobierno
- NIAID NIH HHS
- Subvenciones
- National Institute of Allergy and Infectious Diseases (R56 AI113095); National Institute of Allergy and Infectious Diseases (R37 AI020565); National Institute of Allergy and Infectious Diseases (R21 AI124490); National Institute of Allergy and Infectious Diseases (R01AI-020565); National Institute of Allergy and Infectious Diseases (R01 AI135049); National Institutes of Health (R21 AI124490); National Institutes of Health (R01-AI20565)
Según 4 financiadores declarados.
Publicación
- Publicado
- 2018-12-02 · Clin Exp Allergy · vol. 49 · número 5 · págs. 615–625
- Editorial
- Wiley
- Citado
- 18 citas · más que el 73% de estudios similares · 1.0× el promedio de su campo
- Referencias
- 54 trabajos
- Acceso
- Acceso abierto (copia en repositorio)
- Áreas de investigación
- Food Allergy and Anaphylaxis Research · T-cell and B-cell Immunology · Celiac Disease Research and Management
- Palabras clave
- Flow cytometry, ELISPOT, Immunoglobulin D, Immunoglobulin E, Immunology, Peripheral blood mononuclear cell, Food allergy, Cytometry, Allergy, B cell, Mass cytometry, Biology, Antibody, T cell, Phenotype, In vitro, Immune system, Genetics, Gene
- MeSH
- b-lymphocyte subsets, leukocytes, mononuclear, humans, food hypersensitivity, immunoglobulin e, lymphocyte count, flow cytometry, cluster analysis, case-control studies, immunophenotyping, adult, aged, middle aged, disease management, female, male, young adult, biomarkers, red meat
9 autores
- Kelly M. Cox🇺🇸Carter Center; University of Virginia
- Scott P. Commins🇺🇸University of North Carolina at Chapel Hill
- Brian J. Capaldo🇺🇸University of Virginia
- Lisa J. Workman🇺🇸University of Virginia
- Thomas A.E. Platts‐Mills🇺🇸Carter Center; University of Virginia
- El-ad David Amir
- Josephine A. Lannigan🇺🇸University of Virginia
- Alexander J. Schuyler🇺🇸University of Virginia
- Loren D. Erickson🇺🇸 · autor de correspondenciaCarter Center; University of Virginia
Resumen
El texto original del estudio, en el idioma en que se publicó.
Background
B cells play a critical role in the development and maintenance of food allergy by producing allergen-specific IgE. Despite the importance of B cells in IgE-mediated food allergy, the identity of sIgE-producing human B cells and how IgE is regulated are poorly understood.
Objective
To identify the immunophenotypes of circulating B cells associated with the production of galactose-alpha-1,3-galactose-specific IgE production in patients with red meat allergy.
Methods
B cells in PBMC samples obtained from 19 adults with physician-diagnosed red meat allergy and 20 non-meat allergic healthy controls were assessed by mass cytometry along with a bioinformatics analysis pipeline to identify discrete B cell phenotypes that associated with serum sIgE. Fluorescent flow cytometry was then applied to sort purify discrete B cell subsets, and B cells were functionally evaluated on an individual cell level for the production of sIgE by ELISPOT.
Results
Discrete B cell phenotypes abundant in meat allergic subjects compared to non-meat allergic controls were found in peripheral blood that do not share typical characteristics of classical isotype-switched memory B cells that express high levels of CD27. These B cell subsets shared higher IgD and lower IgM expression levels coupled with CXCR4, CCR6 and CD25 expression. In vitro polyclonal stimulation of purified B cell subsets from meat allergic subjects demonstrated that these subsets were enriched for cells induced to secrete sIgE.
Conclusions and clinical relevance
Circulating B cells display increased abundance of discrete B cell subsets in meat allergic subjects. This observation, coupled with the capacity of individual B cell subsets to produce sIgE following activation, implicates these novel B cell phenotypes in promoting IgE in meat allergy.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.