Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress In Vitro and In Vivo
Rupel K, Zupin L, Colliva A, Kamada A, Poropat A, Ottaviani G, Gobbo M, Fanfoni L, Gratton R, Santoro M, Di Lenarda R, Biasotto M, Zacchigna S
Oxidative medicine and cellular longevity · 55 citas
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Cómo se estudió
- Diseño
- Ensayo clínico controlado (indexado por PubMed)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Universidad u hospital
- International Centre for Genetic Engineering and Biotechnology
- Organización sin fines de lucro
- Associazione Italiana per la Ricerca sul Cancro
- Autores
- Al menos un autor declara un vínculo económico con la industria
- Subvenciones
- Associazione Italiana per la Ricerca sul Cancro (IG2016); Associazione Italiana per la Ricerca sul Cancro (AIRC-IG #2016-19032); Associazione Italiana per la Ricerca sul Cancro (2016 19032); Associazione Italiana per la Ricerca sul Cancro (IG 2016 19032); Associazione Italiana per la Ricerca sul Cancro (19032)
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2018-01-01 · Oxid Med Cell Longev · vol. 2018 · número 1 · pág. 6510159
- Editorial
- Hindawi Publishing Corporation
- Citado
- 79 citas · más que el 95% de estudios similares · 4.2× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 39 trabajos
- Acceso
- Acceso abierto (revista híbrida) · CC-BY
- Áreas de investigación
- Laser Applications in Dentistry and Medicine · Oral health in cancer treatment · Photodynamic Therapy Research Studies
- Palabras clave
- Oxidative stress, Reactive oxygen species, In vivo, Mucositis, In vitro, Viability assay, Inflammation, Antioxidant, Cell biology, Chemistry, Biology, Cancer research, Immunology, Medicine, Biochemistry, Toxicity, Internal medicine, Biotechnology
- MeSH
- neutrophils, keratinocytes, humans, stomatitis, oxidation-reduction, oxidative stress, adult, aged, aged, 80 and over, middle aged, female, male, lasers, semiconductor, low-level light therapy
13 autores
- Katia Rupel🇮🇹University of Trieste
- Luisa Zupin🇮🇹University of Trieste
- Andrea Colliva🇮🇹International Centre for Genetic Engineering and Biotechnology
- Anselmo Jiro Kamada🇧🇷Universidade Federal de Pernambuco
- Augusto Poropat🇮🇹University of Trieste
- Giulia Ottaviani🇮🇹University of Trieste
- Margherita Gobbo🇮🇹University of Trieste
- Lidia Fanfoni🇮🇹University of Trieste
- Rossella Gratton🇮🇹IRCCS Materno Infantile Burlo Garofolo
- Massimo Mattia Santoro🇮🇹University of Padua
- Roberto Di Lenarda🇮🇹University of Trieste
- Matteo Biasotto🇮🇹University of Trieste
- Serena Zacchigna🇮🇹 · autor de correspondenciaUniversity of Trieste; International Centre for Genetic Engineering and Biotechnology
Resumen
El texto original del estudio, en el idioma en que se publicó.
Photobiomodulation (PBM) is emerging as an effective strategy for the management of multiple inflammatory conditions, including oral mucositis (OM) in cancer patients who receive chemotherapy or radiotherapy. Still, the poor understanding of the mechanisms by which the light interacts with biological tissues and the heterogeneity of light sources and protocols employed worldwide significantly limits its applicability. Reactive oxygen species (ROS) are massively generated during the early phases of OM and play a major role in the pathogenesis of inflammation in general. Here, we report the results of a clinical and experimental study, aimed at evaluating the effect of laser light at different wavelengths on oxidative stress in vivo in oncologic patients suffering from OM and in vitro in two cell types abundantly present within the inflamed oral mucosa, neutrophil polymorphonuclear (PMN) granulocytes, and keratinocytes. In addition to standard ROS detection methods, we exploited a roGFP2-Orp1 genetically encoded sensor, allowing specific, quantitative, and dynamic imaging of redox events in living cells in response to oxidative stress and PBM. We found that the various wavelengths differentially modulate ROS production. In particular, the 660 nm laser light increases ROS production when applied either before or after an oxidative stimulus. In contrast, the 970 nm laser light exerted a moderate antioxidant activity both in the saliva of OM patients and in both cell types. The most marked reduction in the levels of ROS was detected in cells exposed either to the 800 nm laser light or to the combination of the three wavelengths. Overall, our study demonstrates that PBM exerts different effects on the redox state of both PMNs and keratinocytes depending on the used wavelength and prompts the validation of a multiwavelength protocol in the clinical settings.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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