Caffeine Protects Skin from Oxidative Stress-Induced Senescence through the Activation of Autophagy
Li YF, Ouyang SH, Tu LF, Wang X, Yuan WL, Wang GE, Wu YP, Duan WJ, Yu HM, Fang ZZ, Kurihara H, Zhang Y, He RR
Theranostics · 155 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Cancer Institute
- Gobierno
- NCI NIH HHS
- Subvenciones
- National Cancer Institute (R01-CA230453)
Según 2 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2018-01-01 · Theranostics · vol. 8 · número 20 · págs. 5713–5730
- Editorial
- Ivyspring International Publisher
- Citado
- 202 citas · más que el 98% de estudios similares · 6.8× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 77 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY-NC
- Áreas de investigación
- Sirtuins and Resveratrol in Medicine · Autophagy in Disease and Therapy · Skin Protection and Aging
- Palabras clave
- Oxidative stress, Senescence, Reactive oxygen species, Autophagy, Caffeine, Cell biology, Chemistry, Gene knockdown, Biology, Biochemistry, Apoptosis
- MeSH
- cells, cultured, skin, animals, humans, mice, skin diseases, disease models, animal, amidines, caffeine, antioxidants, oxidants, treatment outcome, ultraviolet rays, oxidative stress, aging, models, theoretical, autophagy
13 autores
- Yifang Li🇨🇳Jinan University
- Shu‐Hua Ouyang🇨🇳Jinan University
- Long‐Fang Tu🇨🇳Jinan University
- Xi Wang🇨🇳Jinan University
- Wei‐Lin Yuan🇨🇳Jinan University
- Guo-En Wang🇨🇳Jinan University
- Yanping Wu🇨🇳Jinan University
- Wen‐Jun Duan🇨🇳Jinan University
- Hong-Min Yu🇨🇳Jinan University
- Zhong‐Ze Fang🇨🇳Tianjin Medical University
- Hiroshi Kurihara🇨🇳Jinan University
- Youwei Zhang🇺🇸Case Western Reserve University
- Rong‐Rong He🇨🇳Jinan University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Skin cells are vulnerable to oxidative stress-induced senescence, which may lead to abnormal aging or aging-related disorders. Therefore, strategies that can ameliorate oxidative stress-induced senescence are expected to protect skin from damage, holding the promise of treating skin diseases in the clinic. This study aims to investigate whether caffeine, a well-known purine alkaloid, is able to prevent skin from oxidative stress-induced senescence, and to explore the underlying molecular mechanisms. Methods: A free radical inducer 2,2'-Azobis (2-amidinopropane) dihydrochloride (AAPH) was used to induce oxidative stress and cellular senescence in both transformed skin cells and in normal human epidermal keratinocytes (NHEKs). Ultraviolet (UV) irradiation was established as the in vivo oxidative stress model in mouse skin tissues. Cellular senescence was determined by SA β-galactosidase staining, immunofluorescence and western blotting. Activation of autophagy was confirmed by western blotting, immunofluorescence, and transmission electron microscopy. Reactive oxygen species (ROS) detection by commercial kits, gene knockdown by RNA interference (RNAi) and receptor activation/inactivation by agonist/antagonist treatment were applied in mechanistic experiments. Results: We report that AAPH induced senescence in both transformed skin cells and in NHEKs. Similarly, UV irradiation induced senescence in mouse skin tissues. Remarkably, low dose of caffeine (Conclusion: The results reveal that caffeine protects skin from oxidative stress-induced senescence through activating the A2AR/SIRT3/AMPK-mediated autophagy. Our study not only demonstrated the beneficial effect of caffeine using both in vitro and in vivo models, but also systematically investigated the underlying molecular mechanisms. These discoveries implicate the potential of caffeine in the protection of skin disease.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY-NC).
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.