Fish Oil Derived Omega 3 Fatty Acids Suppress Adipose NLRP3 Inflammasome Signaling in Human Obesity
Lee KR, Midgette Y, Shah R
Journal of the Endocrine Society · 43 citas
Etiquetas de revisión
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Cómo se estudió
- Diseño
- Ensayo controlado aleatorizado (clasificado por nuestro filtro de IA)
- Estudiado en
- Personas, más trabajo en animales o laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Institute of Diabetes and Digestive and Kidney Diseases
- Gobierno
- National Center for Advancing Translational Sciences
- Gobierno
- NIDDK NIH HHS
- Gobierno
- NCATS NIH HHS
- Subvenciones
- National Institute of Diabetes and Digestive and Kidney Diseases (P30 DK-019525); National Center for Advancing Translational Sciences (UL1-TR-001878); National Institute of Diabetes and Digestive and Kidney Diseases (K23 DK095913); National Institute of Diabetes and Digestive and Kidney Diseases (DK095913)
Según 4 financiadores declarados.
Publicación
- Publicado
- 2018-12-24 · J Endocr Soc · vol. 3 · número 3 · págs. 504–515
- Editorial
- Endocrine Society
- Citado
- 56 citas · más que el 94% de estudios similares · 3.7× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 60 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY-NC-ND
- Áreas de investigación
- Fatty Acid Research and Health · Adipokines, Inflammation, and Metabolic Diseases · Adipose Tissue and Metabolism
- Palabras clave
- Fish oil, Inflammasome, Adipose tissue, Obesity, Fish <Actinopterygii>, Omega, Chemistry, Endocrinology, Internal medicine, Biochemistry, Medicine, Biology, Fishery, Receptor, Physics
3 autores
- Kailey Roberts Lee🇺🇸Children's Hospital of Philadelphia
- Yasmeen A. Midgette🇺🇸Children's Hospital of Philadelphia
- Rachana D. Shah🇺🇸 · autor de correspondenciaChildren's Hospital of Philadelphia
Resumen
El texto original del estudio, en el idioma en que se publicó.
Context
The NRLP3 inflammasome is a multiprotein danger-sensing complex that serves as a critical link between obesity-related adipose inflammation and insulin resistance and has been shown in animal models to be inhibited by fish oil-derived long chain omega-3 polyunsaturated fatty acids (n-3 PUFA).
Objective
We conducted a clinical trial and in vitro experiments to test our hypothesis that n-3 PUFA suppress NLRP3 inflammasome in human obesity through downregulation of inflammasome gene expression in adipocytes and macrophages.
Design
Placebo-controlled clinical trial and in vitro coculture experiments with primary human adipocytes (from biopsy specimens) and human THP-1 monocyte-derived macrophages treated with eicosapentaenoic acid (EPA) and/or docosahexaenoic acid (DHA) vs vehicle control.
Setting
General community, research laboratory.
Patients and other participants
Obese (body mass index ≥ 30 kg/m2), nondiabetic males and females age 18 to 50. N = 25.
Interventions
Clinical trial: Eight-week treatment with 4 g Lovaza (EPA and DHA) or placebo. Cells culture: EPA and/or DHA at 100 µg/mL or vehicle control in culture medium.
Main outcome measures
Adipose tissue or adipocyte/macrophage mRNA expression of IL-1β and IL-18 and circulating IL-18 levels.
Results
Treatment of obese human subjects with fish oil supplements reduced expression of adipose inflammatory genes including inflammasome-associated IL-18 and IL-1β and circulating IL-18 levels. Both EPA and DHA reduced inflammasome gene expression in obese human adipose and human adipocyte and macrophages.
Conclusions
N-3 PUFA reduce NLRP3 inflammasome in human adipose through downregulation of gene expression in adipocytes and monocytes/macrophages and has potential as nutritional therapeutic agent in prevention of obesity-related inflammation.
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