Discovery of Alpha-Gal-Containing Antigens in North American Tick Species Believed to Induce Red Meat Allergy
Crispell G, Commins SP, Archer-Hartman SA, Choudhary S, Dharmarajan G, Azadi P, Karim S
Frontiers in immunology · 140 citas
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Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Animales
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- U.S. Department of Agriculture
- Gobierno
- National Institutes of Health
- Gobierno
- National Institute of Food and Agriculture
- Gobierno
- National Center for Research Resources
- Gobierno
- NCRR NIH HHS
- Gobierno
- NIH HHS
- Gobierno
- NIGMS NIH HHS
- Gobierno
- NIAID NIH HHS
- Subvenciones
- National Institutes of Health (P41GM10349010); National Institute of Food and Agriculture (2017-67017-26171); National Institute of Food and Agriculture (2016-67030-24576); U.S. Department of Agriculture (2017-67017-26171); National Center for Research Resources (P20 RR016476); U.S. Department of Agriculture (2016-67030-24576); National Institutes of Health (1s10od018530-01); National Institutes of Health (ISIOD108530)
Según 8 financiadores declarados y la declaración de financiación del texto completo.
Publicación
- Publicado
- 2019-05-17 · Front Immunol · vol. 10 · pág. 1056
- Editorial
- Frontiers Media
- Citado
- 175 citas · más que el 99% de estudios similares · 10.9× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 46 trabajos
- Acceso
- Acceso abierto (revista) · CC-BY
- Áreas de investigación
- Food Allergy and Anaphylaxis Research · Allergic Rhinitis and Sensitization · Vector-borne infectious diseases
- Palabras clave
- Amblyomma americanum, Dermacentor variabilis, Tick, Ixodes scapularis, Biology, Antigen, Saliva, Rocky Mountain spotted fever, Microbiology, Immunology, Virology, Ixodidae, Biochemistry
- MeSH
- salivary glands, cells, cultured, saliva, animals, humans, ticks, tick-borne diseases, food hypersensitivity, galactose, immunoglobulin e, allergens, red meat
7 autores
- Gary Crispell🇺🇸University of Southern Mississippi
- Scott P. Commins🇺🇸University of North Carolina at Chapel Hill
- Stephanie A. Archer-Hartman🇺🇸University of Georgia
- Shailesh Kumar Choudhary🇺🇸University of North Carolina at Chapel Hill
- Guha Dharmarajan🇺🇸University of Georgia; Savannah River National Laboratory
- Parastoo Azadi🇺🇸University of Georgia
- Shahid Karim🇺🇸 · autor de correspondenciaUniversity of Southern Mississippi
Resumen
El texto original del estudio, en el idioma en que se publicó.
Development of specific IgE antibodies to the oligosaccharide galactose-α-1, 3-galactose (α-gal) following tick bites has been shown to be the source of red meat allergy. In this study, we investigated the presence of α-gal in four tick species: the lone-star tick (Amblyomma americanum), the Gulf-Coast tick (Amblyomma maculatum), the American dog tick (Dermacentor variabilis), and the black-legged tick (Ixodes scapularis) by using a combination of immunoproteomic approach and, carbohydrate analysis. Anti-α-gal antibodies identified α-gal in the salivary glands of both Am. americanum and Ix. scapularis, while Am. maculatum and De. variabilis appeared to lack the carbohydrate. PNGase F treatment confirmed the deglycosylation of N-linked α-gal-containing proteins in tick salivary glands. Immunolocalization of α-gal moieties to the salivary secretory vesicles of the salivary acini also confirmed the secretory nature of α-gal-containing antigens in ticks. Am. americanum ticks were fed on human blood (lacks α-gal) using a silicone membrane system to determine the source of the α-gal. N-linked glycan analysis revealed that Am. americanum and Ix. scapularis have α-gal in their saliva and salivary glands, but Am. maculatum contains no detectable quantity. Consistent with the glycan analysis, salivary samples from Am. americanum and Ix. scapularis stimulated activation of basophils primed with plasma from α-gal allergic subjects. Together, these data support the idea that bites from certain tick species may specifically create a risk for the development of α-gal-specific IgE and hypersensitivity reactions in humans. Alpha-Gal syndrome challenges the current food allergy paradigm and broadens opportunities for future research.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial (CC BY).
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