In Vitro and in Vivo Studies on the Angiotensin-Converting Enzyme Inhibitory Activity Peptides Isolated from Broccoli Protein Hydrolysate
Dang Y, Zhou T, Hao L, Cao J, Sun Y, Pan D
Journal of agricultural and food chemistry · 49 citas
Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Animales
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Natural Science Foundation of China
- Gobierno
- Science and Technology Department of Zhejiang Province
- Organización sin fines de lucro
- K.C. Wong Magna Fund
- Subvenciones
- Science and Technology Department of Zhejiang Province (2018C02051); Science and Technology Department of Zhejiang Province (2014C02023); National Natural Science Foundation of China (31771945)
Según 3 financiadores declarados.
Publicación
- Publicado
- 2019-06-03 · J Agric Food Chem · vol. 67 · número 24 · págs. 6757–6764
- Editorial
- American Chemical Society
- Citado
- 98 citas · más que el 94% de estudios similares · 3.6× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 32 trabajos
- Acceso
- De pago
- Áreas de investigación
- Protein Hydrolysis and Bioactive Peptides · Insect Utilization and Effects · Biochemical effects in animals
- Palabras clave
- Hydrolysate, In vivo, Peptide, IC50, Chemistry, Inhibitory postsynaptic potential, In vitro, Pharmacology, Renin–angiotensin system, Digestion (alchemy), Enzyme, Angiotensin-converting enzyme, Biochemistry, Pepsin, Endocrinology, Biology, Hydrolysis, Chromatography
- MeSH
- animals, rats, inbred shr, humans, rats, brassica, hypertension, peptidyl-dipeptidase a, peptides, plant proteins, protein hydrolysates, antihypertensive agents, angiotensin-converting enzyme inhibitors, peptide mapping, administration, oral, blood pressure, female
6 autores
- Yali Dang🇨🇳Ningbo University
- Tingyi Zhou🇨🇳Zhejiang Academy of Medical Sciences
- Hao Li🇨🇳Ningbo University
- Jinxuan Cao🇨🇳Ningbo University
- Yangying Sun🇨🇳Ningbo University
- Daodong Pan🇨🇳 · autor de correspondenciaNingbo University; Nanjing Normal University
Resumen
El texto original del estudio, en el idioma en que se publicó.
In the present study we purified and identified peptides from broccoli protein hydrolysates and evaluated their angiotensin-converting enzyme (ACE) inhibitory activity in vitro and hypotensive effect in vivo. Three ACE inhibitory peptides were isolated and identified as IPPAYTK, LVLPGELAK, and TFQGPPHGIQVER, and their inhibitory IC50 values were 23.5, 184.0, and 3.4 μM, respectively. We then investigated the effect of gastrointestinal digestion on ACE inhibitory activity. We detected almost two times the ACE inhibitory activity of the peptide LVLPGELAK following simulated digestion than prior to digestion. LVLPGE and LAK, two novel peptides exhibiting high ACE inhibitory activity, were discovered following digestion and possessed IC50 values of 13.5 and 48.0 μM, respectively. The hypotensive effect of the peptides was assessed after oral administration to spontaneous hypertensive rats (SHRs). We found that LVLPGE and LAK demonstrated a significant hypotensive effect in vivo. Protein from broccoli may thus constitute a potential antihypertensive peptide source.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
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