Processed Meat Protein Promoted Inflammation and Hepatic Lipogenesis by Upregulating Nrf2/Keap1 Signaling Pathway in Glrx-Deficient Mice
Ahmad MI, Zou X, Ijaz MU, Hussain M, Liu C, Xu X, Zhou G, Li C, Li C
Journal of agricultural and food chemistry · 30 citas
Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Animales
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Natural Science Foundation of China
- Subvenciones
- National Natural Science Foundation of China (31530054)
Según 1 financiador declarado.
Publicación
- Publicado
- 2019-07-26 · J Agric Food Chem · vol. 67 · número 32 · págs. 8794–8809
- Editorial
- American Chemical Society
- Citado
- 37 citas · más que el 87% de estudios similares · 1.9× el promedio de su campo
- Referencias
- 64 trabajos
- Acceso
- De pago
- Áreas de investigación
- Genomics, phytochemicals, and oxidative stress · Liver Disease Diagnosis and Treatment · Diet, Metabolism, and Disease
- Palabras clave
- Lipogenesis, KEAP1, Inflammation, Chemistry, Cell biology, Biology, Biochemistry, Lipid metabolism, Immunology, Gene, Transcription factor
- MeSH
- liver, animals, mice, inbred c57bl, mice, knockout, humans, mice, inflammation, cholesterol, triglycerides, cytokines, signal transduction, meat products, female, male, lipogenesis, nf-e2-related factor 2, glutaredoxins, gastrointestinal microbiome, red meat, kelch-like ech-associated protein 1
8 autores
- Muhammad Ijaz Ahmad🇨🇳Nanjing Agricultural University
- Xiaoyou Zou🇨🇳Nanjing Agricultural University
- Muhammad Umair Ijaz🇨🇳Nanjing Agricultural University
- Muzahir Hussain🇨🇳Nanjing Agricultural University
- Congcong Liu🇨🇳Nanjing Agricultural University
- Xinglian Xu🇨🇳Nanjing Agricultural University
- Guanghong Zhou🇨🇳Nanjing Agricultural University
- Chunbao Li🇨🇳 · autor de correspondenciaNanjing Agricultural University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Oxidative stress may play a critical role in the progression of liver disorders. Increasing interest has been given to the associations among diet, oxidative stress, gut-liver axis, and nonalcoholic fatty liver disease. Here, we investigated the effects of processed meat proteins on biomarkers of lipid homeostasis, hepatic metabolism, antioxidant functions, and gut microbiota composition in glutaredoxin1 deficient (Glrx1-/-) mice. The wild-type (WT) and Glrx1-/- mice were fed a soy protein diet (SPD), a dry-cured pork protein diet (DPD), a braised pork protein diet (BPD), and a cooked pork protein diet (CPD) at a dose of 20% of protein for 3 months. Serum and hepatic total cholesterol, serum endotoxin, hepatic liver droplet %, and antioxidant capacity were significantly increased in the CPD fed WT mice. In addition, CPD fed Glrx1-/- mice significantly increased total cholesterol, triacylglycerol, and pro-inflammatory cytokines which are accompanied by higher steatosis scores, intrahepatic lipid accumulation, and altered gene expression associated with lipid metabolism. Furthermore, hepatic gene expression of Nrf2/keap1 signaling pathway and its downstream signaling targets were determined using RT-qPCR. Glrx1 deficiency increased Nrf2 activity and expression of its target genes (GPx, catalase, SOD1, G6pd, and Bbc3), which was exacerbated by intake of CPD. Metagenomic analyses revealed that Glrx1-/- mice fed meat protein diets had higher abundances of Mucispirillum, Oscillibacter, and Mollicutes but lower abundances of Bacteroidales S24-7 group_norank, Blautia, and Anaerotruncus than their wild-type counterparts. In summary, Glrx1 deficiency induced an increase in serum biomarkers for lipid homeostasis, gut microbiota imbalance, and upregulation of Nrf2/Keap1 and antioxidant defense genes, which was aggravated by cooked meat protein diet.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
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