Identification of Cyanidin-3-arabinoside Extracted from Blueberry as a Selective Protein Tyrosine Phosphatase 1B Inhibitor
Tian JL, Liao XJ, Wang YH, Si X, Shu C, Gong ES, Xie X, Ran XL, Li B
Journal of agricultural and food chemistry · 52 citas
Etiquetas de revisión
Solo mecanismosDatos neutrales que nuestra revisión registró sobre cómo se hizo este estudio. Describen el método, nunca si nos gusta el resultado.
Cómo se estudió
- Diseño
- Estudio in vitro o de mecanismos (clasificado por nuestro filtro de IA)
- Estudiado en
- Células o muestras de laboratorio
- Resultado principal
- Solo mecanismos
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- National Natural Science Foundation of China
- Gobierno
- Natural Science Foundation of Liaoning Province
- Gobierno
- Shenyang Science and Technology Bureau
- Universidad u hospital
- Shenyang Agricultural University
- Gobierno
- Key Laboratory of Fruit and Vegetable Processing, Ministry of Agriculture
- Gobierno
- Department of Human Resources of Liaoning Province
- Gobierno
- Liaoning Province
- Gobierno
- Innovative Talent Support Program for Institution of Higher Learning of Liaoning Province
- Subvenciones
- Natural Science Foundation of Liaoning Province (20180550776); Shenyang Agricultural University (880418027); National Natural Science Foundation of China (31671863); Shenyang Science and Technology Bureau (RC170247)
Según 8 financiadores declarados.
Publicación
- Publicado
- 2019-11-19 · J Agric Food Chem · vol. 67 · número 49 · págs. 13624–13634
- Editorial
- American Chemical Society
- Citado
- 73 citas · más que el 92% de estudios similares · 2.8× el promedio de su campo
- Impacto
- Entre el 10 % más citado de su campo
- Referencias
- 30 trabajos
- Acceso
- De pago
- Áreas de investigación
- Protein Tyrosine Phosphatases · Microbial Metabolites in Food Biotechnology · Seaweed-derived Bioactive Compounds
- Palabras clave
- Oleanolic acid, Chemistry, Biochemistry, Protein tyrosine phosphatase, IC50, Phosphatase, Protein kinase B, GSK-3, Enzyme, Pharmacology, Phosphorylation, Biology, In vitro
- MeSH
- humans, fruit, anthocyanins, glucosides, plant extracts, enzyme inhibitors, molecular structure, kinetics, protein tyrosine phosphatase, non-receptor type 1, phosphatidylinositol 3-kinases, molecular docking simulation, blueberry plants
9 autores
- Jinlong Tian🇨🇳Shenyang Agricultural University
- Xiao-Jun Liao🇨🇳Beijing Advanced Innovation Center for Food Nutrition and Human Health; China Agricultural University
- Yuehua Wang🇨🇳Shenyang Agricultural University
- Xu Si🇨🇳Shenyang Agricultural University
- Chi Shu🇨🇳Shenyang Agricultural University
- Er Sheng Gong🇨🇳Shenyang Agricultural University
- Xu Xie🇨🇳Shenyang Agricultural University
- Xu-Long Ran🇨🇳Shenyang Agricultural University
- Bin Li🇨🇳 · autor de correspondenciaShenyang Agricultural University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Protein tyrosine phosphatase 1B (PTP1B) is an important target for type 2 diabetes. PTP1B inhibitors can reduce blood glucose levels by increasing insulin sensitivity. Anthocyanins often play a hypoglycemic effect, but the research about them have mainly focused on glucosidase. At present, the research about protein tyrosine phosphatase 1B (PTP1B) target is less, and the corresponding molecular mechanism is still unclear. Therefore, in this present study, anthocyanins isolated from blueberry were used to study the inhibitory activity on PTP1B. The isolated cyanidin-3-arabinoside (Cya-3-Ara) exhibited a better inhibitory activity with IC50 = 8.91 ± 0.63 μM, which was higher than the positive control (oleanolic acid, IC50 = 13.9 ± 1.01 μM), and the mechanism of PTP1B inhibition was reversible mixed pattern. The structure-activity relationship (SAR) between anthocyanins and PTP1B inhibition was investigated. The enzyme activity inhibition and molecular docking showed that anthocyanins had high selectivity for PTP1B inhibition. Further study showed that Cya-3-Ara could promote glycogen synthesis through ameliorating PTP1B-involved IRS-1/PI3K/Akt/GSK3β pathways. Cya-3-Ara could also be regarded as a synergistic inhibitor (CI ≤ 0.54) of oleanolic acid to obtain a better inhibitory effect on PTP1B. Taken together, our study clearly illustrates the SAR between anthocyanins and PTP1B inhibition and the mechanism of Cya-3-Ara in the insulin signaling pathway.
Resumen vía Europe PMC. Los derechos de autor siguen siendo de los autores o la editorial.
Confianza de la comunidad
Cargando…
¿Cuánto confías en los hallazgos de este estudio?
Comentarios
¿Algo está mal aquí?
Señala este estudio si su información, etiquetas o financiación parecen incorrectas. Un editor revisa cada señalamiento.
Información educativa sobre investigación publicada. No es consejo médico ni una recomendación para empezar o dejar nada.