Gelatin hydrogels with eicosapentaenoic acid can prevent osteoarthritis progression in vivo in a mouse model
Tsubosaka M, Kihara S, Hayashi S, Nagata J, Kuwahara T, Fujita M, Kikuchi K, Takashima Y, Kamenaga T, Kuroda Y, Takeuchi K, Fukuda K, Takayama K, Hashimoto S, Matsumoto T, Niikura T, Tabata Y, Kuroda R
Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 22 citas
Cómo se estudió
- Diseño
- Estudio en animales (clasificado por nuestro filtro de IA)
- Estudiado en
- Animales
- Resultado principal
- Marcadores de salud y funcionamiento
Quién lo pagó
- Financiación
- Financiación independiente
- Gobierno
- Japan Society for the Promotion of Science
- Subvenciones
- Japan Society for the Promotion of Science (18K09105); Japan Society for the Promotion of Science (20K09147)
Según 1 financiador declarado.
Publicación
- Publicado
- 2020-04-09 · J Orthop Res · vol. 38 · número 10 · págs. 2157–2169
- Editorial
- Wiley
- Citado
- 31 citas · más que el 89% de estudios similares · 2.4× el promedio de su campo
- Referencias
- 42 trabajos
- Acceso
- Acceso abierto (copia en repositorio)
- Áreas de investigación
- Osteoarthritis Treatment and Mechanisms · Inflammatory mediators and NSAID effects · Veterinary Equine Medical Research
- Palabras clave
- Eicosapentaenoic acid, Gelatin, Self-healing hydrogels, Chemistry, In vivo, Corn oil, Osteoarthritis, Polyunsaturated fatty acid, Hyaluronic acid, Pharmacology, Medicine, Fatty acid, Biochemistry, Pathology, Food science, Biology, Biotechnology, Anatomy
- MeSH
- cartilage, articular, animals, mice, inbred c57bl, humans, osteoarthritis, synovitis, disease progression, eicosapentaenoic acid, gelatin, hydrogels, random allocation, drug evaluation, preclinical, micelles, male, primary cell culture, biomarkers
18 autores
- Masanori Tsubosaka🇯🇵Kobe University
- Shinsuke Kihara🇯🇵Kobe University
- Shinya Hayashi🇯🇵 · autor de correspondenciaKobe University
- Junpei Nagata🇯🇵Kyoto University
- Toshie Kuwahara🇯🇵Kyoto University
- Masahiro Fujita🇯🇵Kobe University
- Kenichi Kikuchi🇯🇵Kobe University
- Yoshinori Takashima🇯🇵Kobe University
- Tomoyuki Kamenaga🇯🇵Kobe University
- Yuichi Kuroda🇯🇵Kobe University
- Kazuhiro Takeuchi🇯🇵Kobe University
- Koji Fukuda🇯🇵Kobe University
- Koji Takayama🇯🇵Kobe University
- Shingo Hashimoto🇯🇵Kobe University
- Tomoyuki Matsumoto🇯🇵Kobe University
- Takahiro Niikura🇯🇵Kobe University
- Yasuhiko Tabata🇯🇵Kyoto University
- Ryosuke Kuroda🇯🇵Kobe University
Resumen
El texto original del estudio, en el idioma en que se publicó.
Eicosapentanoic acid (EPA) is an antioxidant and omega-3 polyunsaturated fatty acid that reduces inflammatory cytokine production. Gelatin hydrogel can be used as a carrier of a physiologically active substance that release it gradually for an average of ~3 weeks. Therefore, this study aimed to clarify the effect of EPA-incorporating gelatin hydrogels on osteoarthritis (OA) progression in vivo. Ten-week-old male C57BL/6J mice were randomly divided into six groups (n = 6): Sham, destabilization of the medial meniscus (DMM), Corn: DMM + 2 µL corn oil, EPA injection alone (EPA-I): DMM + 2 µL corn oil + 125 μg/μL EPA, Gel: DMM + gelatin hydrogels, and EPA-G: DMM + 125 μg/μL EPA-incorporating gelatin hydrogels. The mice were euthanized at 8 weeks after DMM or Sham surgery, and subjected to histological evaluation. Matrix-metalloproteinases-3 (MMP-3), MMP-13, interleukin-1β (IL-1β), p-IKK α/β, CD86, and CD163 protein expression in the synovial cartilage was detected by immunohistochemical staining. F4/80 expression was also assessed using the F4/80 score of macrophage. Histological score was significantly lower in EPA-G than in EPA-I. MMP-3-, MMP-13-, IL-1β-, and p-IKK α/β-positive cell ratio was significantly lower in EPA-G than in EPA-I. However, CD86- and CD163-positive cell ratio was not significantly different between EPA-I and EPA-G. The average-sum F4/80 score of macrophage in EPA-G was significantly lower than that in EPA-I. EPA-incorporating gelatin hydrogels were shown to prevent OA progression in vivo more effectively than EPA injection alone. Our results suggested that intra-articular administration of controlled-release EPA can be a new therapeutic approach for treating OA.
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